ASGPR1,唾液酸糖蛋白受体1抗体

产品编号HZ-4119R
英文名称ASGPR1
中文名称唾液酸糖蛋白受体1抗体
别 名ASGR; Asgr1; ASGPR1; Asgr; Asgr-1: MGC108731; RATRHL1; RHL1; ASGR1_HUMAN; Asialoglycoprotein receptor 1; ASGP-R 1; ASGPR 1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1.
规格价格0.1ml 0.2ml 
说 明 书0.1ml 0.2ml
研究领域免疫学 结合蛋白 糖蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
ASGPR1,唾液酸糖蛋白受体1抗体分 子 量32kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ASGPR1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ASGPR1,唾液酸糖蛋白受体1抗体PubMedPubMed
产品介绍background:
ASGR is a heterooligomeric receptor that is abundantly expressed on the sinusoidal surface of the hepatic plasma membrane. It is an endocytic receptor that rapidly binds and internalizes galactose-terminated glycoproteins (asialoglycoproteins or ASGP) from the circulation. The mouse ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The aa sequence of mouse ASGPR1 ECD is 89% and 79% identical to the ASGPR1 ECD of rat and human, respectively.

Function:
Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Subunit:
Interacts with LASS2.

Subcellular Location:
Membrane; Single-pass type II membrane protein.

Tissue Specificity:
Expressed exclusively in hepatic parenchymal cells.

Post-translational modifications:
Phosphorylated on a cytoplasmic Ser residue.

Similarity:
Contains 1 C-type lectin domain.

ASGPR1,唾液酸糖蛋白受体1抗体Database links:
UniProtKB/Swiss-Prot: P07306.2 human
UniProtKB/Swiss-Prot: P34927.4 mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ASGR1 Polyclonal Antibody, Unconjugated(bs-4119R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ASGR1 Polyclonal Antibody, Unconjugated(bs-4119R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ASGPR1 Polyclonal Antibody, Unconjugated(bs-4119R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining