Recombinant Human GDNF
 
Catalog Number:
AF-450-10
Synonyms:
Glial-Derived Neurotrophic, Factor, ATF-1
Description:
 
GDNF是一种由二硫键连接的同源二聚体神经营养因子，结构上与Artemin、Neurturin和Persephin相关。这些蛋白属于生长因子的半胱氨酸结(cysteine-knot)超家族，具有稳定的二聚体蛋白结构。GDNF通过一个多组份受体系统传递信号，该系统由RET(神经酪氨酸激酶)和四个GFR(1-4)受体之一组成。GDNF特异性促进多巴胺摄取，以及中脑神经元的存活和形态分化。用帕金森氏症小鼠模型证实，GDNF能够改善动作徐缓、肌强直和姿势不稳等症状。具有功能的小鼠GDNF配体是由二硫键连接而成的同源二聚体，含两条15.kDa的多肽链单体。每个单体均有七个保守的半胱氨酸残基，其中一个(Cys 101)用于形成链间二硫键桥，而其它几个参与分子内环，即半胱氨酸结的形成。生产过程中的所有试剂均为非动物源性。
AA Sequence:
MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Source:
E.coli
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 was determined by the proliferation of rat C6 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
 
 
 
 
Cross Reactivity:
Human
Research Interests:
Angiogenesis/ Cardiovascular
Neurobiology
Stem Cells & Differentiation
TGF-β Superfamily
 
 
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.