CXCL4,血小板因子4抗体

产品编号HZ-2548R
英文名称CXCL4
中文名称血小板因子4抗体
别 名C-X-C motif chemokine 4; Chemokine (C X C motif) ligand 4; Chemokine (CXC motif) ligand 4; CXCL 4; CXCL4; Iroplact; MGC138298; Oncostatin A; Oncostatin-A; OncostatinA; PF 4; PF-4; PF4; Platelet factor 4; PLF4_HUMAN; SCYB 4; SCYB4; short form; Small inducible cytokine subfamily member 4.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 生长因子和激素 趋化因子 血管内皮细胞
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Cow, Rabbit,
CXCL4,血小板因子4抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量11kDa
细胞定位分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CXCL
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CXCL4,血小板因子4抗体PubMedPubMed
产品介绍background:
Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7 transmembrane, G protein coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines. CXC chemokines are further subdivided into ELR and non ELR types based on the presence or absence of a glu leu arg sequence adjacent and N terminal to the CXC motif.

Function:
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.

Subunit:
Homotetramer.

Subcellular Location:
Secreted.

CXCL4,血小板因子4抗体Similarity:
Belongs to the intercrine alpha (chemokine CxC) family.

Gene ID:
5196

Database links:
Entrez Gene: 5196 Human
Entrez Gene: 360918 Rat
Omim: 173460 Human
SwissProt: P02776 Human
SwissProt: P06765 Rat
Unigene: 81564 Human
Unigene: 6800 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CXCL4/PF4 Polyclonal Antibody, Unconjugated(HZ-2548R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining