The Human Gastric Cancer EpiTect Methyl II Signature PCR Array profiles the methylation status of 22 tumor suppressor gene promoters whose hypermethylation has been reported in the literature to occur frequently in a variety of gastric tumors. Profiling tumor samples with these arrays may help correlate CpG island methylation status with biological phenotypes. The results may also provide insights into the molecular mechanisms and biological pathways behind gastric cancer development and progression, as well as its pathology. With a simple restriction enzyme digestion and real-time PCR, research studies can analyze the methylation status of 22 different gastric cancer genes with this DNA methylation PCR array.

Both 96-well and 384-well ( 4 X 96 ) formats are available.

The EpiTect Methyl II PCR Arrays use the MethylScreen™ Technology provided under license from Orion Genomics, LLC

胃癌甲基化PCR芯片用于研究文献中已经报道的在各种各样的胃肿瘤经常发生甲基化的22个肿瘤抑制基因启动子的甲基化状态。利用这些芯片分析细胞或新鲜组织基因组DNA样本有助于关联CpG岛甲基化状态与生物表型。结果还可以提供洞察胃癌发生和进展的分子机制和生物学通路，及其病理。利用这个芯片，通过简单的限制性内切酶消化和实时定量PCR，就可以研究分析22个不同胃癌基因的启动子甲基化状态。

提供96-孔板和384-孔板( 4 X 96 )。

Apoptosis & Anti-Apoptosis: FHIT, SFN (14-3-3 sigma).

Cell Adhesion & Migration: CDH1, CDH13, TIMP3.

Cell Cycle, Growth, Differentiation & Development: CCND2 (cyclin D2), CDKN2A, CHFR, FHIT, KLF4, MLH1, RARB, RASSF1, RB1, SFN.

DNA Damage Repair: MLH1, RASSF1, RB1.

DNA Methyltansferase: MGMT (AGT).

Extracellular Matrix Remodeling: LOX, TIMP3.

Histidine Triad Gene Family: FHIT.

Inflammation: PTGS2 (COX2).

Mitogenesis: PTGS2 (COX2).

Nuclear Receptors: RARB.

Proteases Inhibitors: TIMP3.

Protein Kinase: PRKCDBP.

Retinoid Acid Signaling: RARB.

Transcription Factors: KLF4, RB1, RUNX3.

Tumor Suppressor Gene Candidates: CHFR, MLH1, PRKCDBP (SRBC), RASSF1, RB1, RUNX3, SFN, TMEFF2 (HPP1, TPEF).

WNT Signaling: DKK2, SFRP2, SFRP5