FITC标记的转录调控因子RFX4抗体
产品名称： FITC标记的转录调控因子RFX4抗体
英文名称： Anti-RFX4/FITC
产品编号： HZ-11943R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-RFX4/FITC Conjugated antibody
FITC标记的转录调控因子RFX4抗体
| 英文名称 | Anti-RFX4/FITC |
| 中文名称 | FITC标记的转录调控因子RFX4抗体 |
| 别 名 | NYD SP10; Regulatory factor X 4; Regulatory factor X, 4 (influences HLA class II expression); Regulatory factor X4 isoform E; Regulatory factor X4 isoform F; Regulatory factor X4, isoform c; Regulatory factor X4, isoform d; Transcription factor RFX4; Winged helix transcription factor RFX4 variant 3; RFX4_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 神经生物学 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep, Guinea Pig, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 83kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RFX4 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: EP and EP-like sites are regulatory enhancer elements found in the promoters of several viral and mammalian genes which, in humans, include the MIF-1 binding site (MIE) of the c-Myc gene, the X box of MHC class II promoters and a binding site in the PCNA (proliferating cell nuclear antigen) promoter. The EP-like sites present in the X box of MHC class II promoters are distinctly nonpalindromic sequences that contain only a single EP-homologous half-site. The EP-like element is bound by a ubiquitous nuclear protein complex that consists of homo- and heterodimers involving the RFX1, RFX2, RFX3, RFX4 and RFX5 proteins. The RFX proteins represent an essential class II transcription factor family that share several conserved regions, including a centrally located DNA-binding domain (DBD) and a C-terminal D region that facilitates dimerization. RFX4 is a 735 amino acid nuclear protein that, via interactions with other RFX proteins, can bind DNA and is thought to activate the transcription of target genes. Four isoforms, each of which exhibit different tissue specificity, exist due to alternative splicing events. Function: RFX4 is a transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX4 is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. RFX4 may be a transcriptional repressor rather than a transcriptional activator.This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. This protein may be a transcriptional repressor rather than a transcriptional activator. Three transcript variants encoding different isoforms have been described for this gene. Subunit: Homodimer. Heterodimer with RFX2 and RFX3. Binds DNA. Subcellular Location: Nuclear Tissue Specificity: Isoform 4 is testis-specific. Isoform 1 is expressed in brain and gliomas. Isoform 2 and isoform 3 are testis-specific (at protein level). Isoform 1 is expressed in brain (at protein level). Isoform 3 is expressed at a higher level in adult testes and ejaculated spematozoa than in fetal testes. Similarity: Belongs to the RFX family. Contains 1 RFX-type winged-helix DNA-binding domain. Database links: Entrez Gene: 5992 Human Entrez Gene: 71137 Mouse Entrez Gene: 500818 Rat Omim: 603958 Human SwissProt: Q33E94 Human SwissProt: Q7TNK1 Mouse Unigene: 388827 Human Unigene: 79070 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
EP和EP样位点是在几种病毒和哺乳动物基因的启动子中发现的调节增强子元件，在人类中，这些启动子包括c-Myc基因的MIF-1结合位点(MIE)、MHC II类启动子的X盒和PCNA(增殖细胞核抗原)中的结合位点促进。r.存在于MHCⅡ类启动子X盒中的EP样位点是明显非回文序列，仅包含单个EP同源半位点。EP样元件由普遍存在的核蛋白复合物结合，该复合物由涉及RFX1、RFX2、RFX3、RFX4和RFX5蛋白的同源和异源二聚体组成。RFX蛋白代表一个基本II类转录因子家族，该家族共享几个保守区域，包括位于中心的DNA结合域（DBD）和促进二聚化的C端D区域。RFX4是一种735个氨基酸的核蛋白，通过与其他RFX蛋白的相互作用，可以结合DNA，并被认为激活靶基因的转录。四种异构体，每个都表现出不同的组织特异性，由于选择性剪接事件存在。