产品编号:
500-P201GBt
来源:
Polyclonal Goat
制备:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mCXCL16. Anti-Murine CXCL16 specific antibody was purified by affinity chromatography and then biotinylated.
免疫原:
E.coli derived Recombinant Murine CXCL16 (PeproTech catalog #250-28)
应用:
ELISA
Direct
To detect mCXCL16 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mCXCL16.
Sandwich
To detect mCXCL16 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine CXCL16 (500-P201G) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mCXCL16.
Western Blot
To detect mCXCL16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mCXCL16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
研究领域: 趋化 炎症 创伤修复 免疫系统
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