* 减少多次实验间的差异对实验结果造成的影响

Springbio高通量抗体芯片的特点
* 高密度，一次可检测720个蛋白分子，涉及多种生物学功能
* 特异性高，芯片中的每一种抗体均为WB、IP和IHC级别

图示: Springbio抗体芯片基于抗体抗原特异性结合的原理，

芯片上的抗体能够与样品中的蛋白结合，最终通过荧光信号反应样品中蛋白含量。

 

芯片检测的蛋白涉及多种重要生物学功能

 

Epithelial Membrane Antigen (EMA / CA15-3 / MUC-1), Epithelial Specific Antigen / Ep-CAM, Fli-1, GCDFP-15 (Gross Cystic Disease Fluid Protein-15), Glicentin, Granulocyte (Myeloid cell Marker), Hepatocyte, IPO-38 (Proliferation Marker), Ki-67, L1 Cell Adhesion Molecule, LRP / MVP (Major Vault Protein), MAGE-1, MART-1 / Melan-A (Melanoma Marker), Milk Fat Globule Membrane protein, Human (HMFG1), Muc-1, Mucin 2 (MUC2), Mucin 3 (MUC3), Mucin 5AC (MUC5AC) / Gastric Mucin, Nucleophosmin (NPM), p170 / p-Glycoprotein / MDR (Anti-Hamster), p170 / p-Glycoprotein / MDR, Placental Alk. Phosphatase (PLAP), Progesterone Receptor (Serine190 Phospho-specific), Progesterone Receptor (Serine294 Phospho-specific), Progesterone Receptor, Prostate Specific Acid Phosphatase (PSAP), Prostate Specific Antigen (PSA), pS2 / pNR-2 Estrogen-Regulated Protein, PSCA (Prostate Stem Cell Antigen), PTEN / MMAC1, Renal Cell Carcinoma Marker (gp200), S100 Protein, S100A4, Surfactant Protein A (SP-A), Surfactant Protein B (Mature), Surfactant Protein B (Precursor), Synaptophysin, TAG-72 / CA 72-4, Tartrate Resistant Acid Phosphatase (TRAP), Thomsen-Friedenreich Antigen, Thyroglobulin, Topoisomerase II alpha, Topoisomerase II beta, TTF-1 (Thyroid Transcription Factor-1), Tyrosinase, Tyrosinase Related Protein 75 (TRP75 / gp75)

 

Cell Cycle: APC11(Anaphase-Promoting Complex), APC2 (Anaphase-Promoting Complex), CDC14A Phosphatase, CDC25A, CDC25B, CDC25C, CDC34, CDC37, CDC47 / MCM7, CDC6, CDC7 phosphatase, Cdh1, cdk1 / p34cdc2, Cdk2, Cdk3, Cdk4, Cdk5, Cdk6, cdk7 / p40MO15 / CAK, Cdk8, Chk1, Chk2, Cullin-1 (CUL-1), Cullin-2 (CUL-2), Cullin-3 (CUL-3), Cyclin A, Cyclin B1, Cyclin C, Cyclin D1 / Bcl-1, Cyclin D2, Cyclin D3, Cyclin E, Cyclin E2, Ki-67, Mitochondria, p107, p130 / Rb2, p130Cas, p14ARF / p16Beta, p15INK4b, p16INK4a, p18INK4c, p19ARF, p19INK4d, p19Skp1, p21WAF1, p27Kip1, p35nck5a, p53, p57Kip2, p73, p73alpha , p73alpha/Beta, PCNA (Proliferating Cell Nuclear Antigen), ROC.

 

DNA Damage Repair: DNA Ligase I, DNA Polymerase beta, DNA Polymerase gamma (C-Terminal) (CD7), DNA Primase (p49), DNA Primase (p58), DNA-PKcs, ERCC1, Exo1, Histone H1, Ku (p70 / p80), Ku (p70), Ku (p80), MCM2, MCM5, MDM2, MLH1, RAD1, Rad51, Ribonucleoprotein (RNP), RPA / p32 (Replication Protein A), RPA / p70 (Replication Protein A), RuvA, RuvB, RuvC, sm (Smith Antigen), TdT (Terminal Deoxyribonucleotidyl Transferase), Thymidylate Synthase, Thymine Glycols, Topoisomerase II alpha, Topoisomerase II beta, Xanthine Oxidase / Aldehyde Oxidase, XPA, XPF, XPG / ERCC5, XRCC1 (X-Ray Repair Cross Complementing Protein1), XRCC2 (X-Ray Repair Cross Complementing Protein2).

 

Stress Response: HDJ-2 / DNAJ, Heat Shock Factor 1 (HSF1), Heat Shock Factor 2 (HSF2), Heat Shock Protein 27 (HSP27), Heat Shock Protein 60 (hsp60), Heat Shock Protein 70 (HSP70), Heat Shock Protein 75 / hsp75 / TRAP-1, Heat Shock Protein 90alpha / hsp90alpha (also known as hsp86), Heat Shock Protein 90Beta / hsp90Beta (also known as hsp84), HIF-1alpha (Hypoxia-Inducible Factor-1alpha), HIF-1beta (Hypoxia-Inducible Factor-1beta), Superoxide Dismutase (Cu/Zn).

 

Cell Senescence: Acinus, AIF (Apoptosis Inducing Factor), ARC (Apoptosis Repressor with CARD), Ask1 / MAPKKK5, BAG-1/RAP46/HAP1, Bak, Bax, Bcl10 / CIPER / CLAP / mE10, Bcl-2alpha, Bcl-6, Bcl-X, Bcl-XL, Bim / BOD (Bcl-2-related Ovarian Death Gene), Caspase 1 / Interleukin-1Beta Converting Enzyme (ICE), Caspase 2, Caspase 3 (CPP32), Caspase 5, Caspase 6 (Mch 2), Caspase 7 (Mch 3), Caspase 8 (FLICE), Caspase 9 / LAP6, Cathepsin D, CD137 (4-1BB), CD14, CD27, CD40, CD95 (Fas), CIDE-A, CIDE-B, D4-GDI, Daxx, DcR1, DcR2 / TRAIL-R4 / TRUNDD, DFF40 (DNA Fragmentation Factor 40) / CAD, DFF45 (DNA Fragmentation Factor 45) / ICAD (Anti-Mouse), DR3 / Apo3 / WSL-1 / TRAMP / LARD, DR5, FADD (FAS-Associated Death Domain-containing Protein), Fas Ligand, FLIP, Granzyme B, I-FLICE / CASPER (Inhibitor of FLICE), MAP-kinase-Activating Death Domain protein / MADD, Mcl-1, PARP (Poly ADP-Ribose Polymerase), PARP (Poly ADP-Ribose Polymerase), Prohibitin (Mitochondrial Marker), RAIDD, SODD (Silencer of Death Domain), Survivin, TR2 / HVEM, TRADD (TNF-R1-Associated Death Domain).

 

Adhesion Molecule: Cadherin Pan, Cadherin-E / E-Cadherin, Cadherin-P / P-cadherin, CD100 / Leukocyte Semaphorin, CD106 / VCAM, CD138, CD155 / PVR (Poliovirus Receptor), CD165 (gp37), CD18 (Integrin Beta-2), CD26 / DPP IV / ADA-Binding Protein, CD29 (Integrin Beta-1), CD31 / PECAM-1, CD38, CD48, CD50 / ICAM-3, CD54 / ICAM-1, CD56 / NCAM-1, CD57 (Natural Killer Cell Marker), CD6, CD62E / ELAM, CD81 / TAPA-1.

 

Angiogeniss: CD105 / Endoglin / TGF-beta1/3 Receptor, CD31 / PECAM-1, CD34 TypeⅡ(Endothelial Cell Marker), Endostatin, Flk-1 / KDR / VEGFR2, Flt-1 / VEGFR1, Flt-4, Heparan Sulfate Proteoglycan / Perlecan, Thrombospondin (TSP), Thymidine Phosphorylase / PD-ECGF, Vascular Endothelial Growth Factor (VEGF).

 

Cadherin,Catenins and Claudin: Cadherin Pan, Cadherin-E / E-Cadherin, Cadherin-P / P-cadherin, Catenin-beta, Catenin-gamma, Catenin-alpha, Claudin 2, Claudin 3, Claudin 4, Claudin 5, Claudin-1, Connexin 43.

 

Cell Junctions: Cadherin Pan, Cadherin-E / E-Cadherin, Cadherin-P / P-cadherin, Catenin-beta, Catenin-gamma, Catenin-alpha, Claudin 2, Claudin 3, Claudin 4, Claudin 5, Claudin-1, Connexin 43.

 

Cytoskeletal Proteins: Actin Beta, Clathrin , Light Chain, Ezrin / p81 / 80K / Cytovillin, Fascin, Filaggrin, Filamin, Gαi1 G-Protein, Involucrin, Keratin 10 / 13, Keratin 10, Keratin 14, Keratin 15, Keratin 16, Keratin 18 (Phospho-specific Ser33), Keratin 18, Keratin 19, Keratin 20, Keratin 5 / 6 / 18, Keratin 5 / 8, Keratin 6, Keratin 8 (phospho-specific Ser431), Keratin 8 (phospho-specific Ser73), Keratin 8 / 18, Keratin 8, Keratin High Molecular Weight, Keratin Low Molecular Weight, Keratin Multi, Keratin Pan, Moesin, Neurofilament (140-160kDa), Neurofilament (200kDa), Neurofilament (68kDa), Paxillin, L-Plastin, Tubulin-alpha, Tubulin-Beta, Vimentin, Vinculin.

 

Extracellular Matrix Proteins: Collagen II, Collagen IV, Collagen IX, Collagen VII, Endothelial Marker (Small Blood Vessels), Fibrillin-1, Fibronectin, Laminin B1 / Beta1, Laminin B2 / gamma1, Laminin Receptor, Laminin-s, Osteopontin, Tenascin.

 

Proteases & Inhibitors: MMP-1 (Collagenase-1), MMP-10 (Stromelysin-2), MMP-11 (Stromelysin-3), MMP-13 (Collagenase-3), MMP-14 / MT1-MMP, MMP-15 / MT2-MMP, MMP-2 (72kDa Collagenase IV), MMP-23, MMP-3 (Stromelysin-1), MMP-7 (Matrilysin), MMP-9 (92kDa Collagenase IV), PR3 (Proteinase 3), TACE (TNF-alpha converting enzyme) / ADAM17, TIMP-1, TIMP-2. Proteolysis: Cullin-1 (CUL-1), Cullin-2 (CUL-2), Cullin-3 (CUL-3), Ubiquitin.

 

EGFR family and Receptor Tyrosine Kinase: Amphiregulin, CD115 / c-fms / CSF-1R / M-CSFR, CD117 / c-Kit / SCF-Receptor, c-erbB-2 / HER-2 / neu(21N), c-erbB-3 / HER-3, c-erbB-4 / HER-4, Epidermal Growth Factor Receptor (EGFR), Heregulin / NDF / GGF / Neuregulin.

 

Insulin homeostasis: Amylin Peptide, Glucose-Regulated Protein 94 (Grp94) / gp96, GLUT-1, GLUT-3, Insulin, Insulin Receptor (alpha-Subunit), Insulin Receptor Substrate-1 (IRS-1), Int-2 Oncoprotein.

 

Transcriptional Regulators: AP-2 Transcription Factor, CBP (CREB-Binding Protein), c-fos, CITED1 (CBP/p300-interacting transactivator 1) / Melanocyte-specific protein 1, c-jun, c-myb / v-myb, c-myc, CREB, DP-1 Transcription Factor, DP-2 Transcription Factor, E2F-1 Transcription Factor, E2F-2 Transcription Factor, E2F-3 Transcription Factor, E2F-4 Transcription Factor, E2F-5 Transcription Factor, Fli-1, Fra2, FRA2 (Fos Related Antigen2), GRIP1 (Glucocorticoid Receptor Interacting Protein 1), HDAC1, Hepatic Nuclear Factor-3Beta (HNF-3Beta), Hepatocyte Factor homologue 4 (HFH-4), HIF-1alpha (Hypoxia-Inducible Factor-1alpha), HIF-1beta (Hypoxia-Inducible Factor-1beta), Microphthalmia (Transcription Factor) (MiTF) , NF kappa B / p50, NF kappa B / p65 (Rel A), 1-Oct, 2-Oct, p300 / CBP, SREBP-1 (Sterol Regulatory Element Binding Protein-1), Stat1, Stat3, Stat5, Stat5a, Stat5b, Stat6 (IL-4 Stat), TTF-1 (Thyroid Transcription Factor-1), Vitamin D Receptor (VDR).

 

APC (Adenomateous Polyposis Coli), BRCA2, CD84, FHIT (Fragile Histidine Triad)., MGMT (O-methylguanine-DNA methyltransferase), nm23 / NDP Kinase, p107, p130 / Rb2, p130Cas, p14ARF / p16Beta, p15INK4b, p16INK4a, p21WAF1, p27Kip1, p53, Retinoblastoma (Rb) (Phospho-specific Serine), Retinoblastoma (Rb), SMAD4 / DPC4, von Hippel-Lindau (vHL) Protein, Wilm Tumor Protein (WT1).

 

Hematopoiesis Diferentiation: CD1a, CD1b, CD32 / Fcgamma Receptor II, CD35 / CR1 (Follicular Dendritic Cell Marker), CD36 / GPIIIb / GPIV, CD41, CD42b (GPIb), CD45RB, CD46, CD47, CD5, CD59 / MACIF / MIRL / Protectin, CD61 / Platelet Glycoprotein IIIA, CD63, CD68 (Macrophage Marker), CD79b (BCR-Ig beta), CD9 (Motility-Related Protein-1), CD94, CD98 / FRP1, CDw78, Factor VIII Related Antigen / von Willebrand Factor, Factor XIII A, Granulocyte (Myeloid cell Marker), Kappa Light Chain, Lambda Light Chain, Lewis A (Blood Group Antigen), Macrophage / L1 Protein / Calprotectin, MHC I (HLA-A), MHC I (HLA-A, B, C), MHC I (HLA-A25 and HLA-Aw32), MHC I (HLA-B), MHC II (HLA-DP), MHC II (HLA-DQ), MHC II (HLA-DR and DP), MHC II (HLA-DR), MHC II (HLA-DR)1α, Microglobulin Beta2, Myeloid Specific marker, p95vav, Pax-5, Plasma Cell marker, Plasminogen.

 

Stem Cell Diferentiation: Activin Receptor Type II (actRII), Alkaline Phosphatase (AP), Alpha Fetoprotein, CD117 / c-Kit / SCF-Receptor, CD29 (Integrin Beta-1), CD30 (Reed-Sternberg Cell Marker), CD34 TypeⅡ(Endothelial Cell Marker), CD38, CD4, CD44 Std. / HCAM, CD45 / T200 / LCA, CD56 / NCAM-1, CD8, CD90 / Thy-1, Collagen II, Collagen IV, Flk-1 / KDR / VEGFR2, GFAP (Glial Fibrillary Acidic Protein), GFAP (Glial Fibrillary Acidic Protein), Glucagon, Heparan Sulfate Proteoglycan / Perlecan, Insulin, Keratin 10 / 13, Keratin 10, Keratin 14, Keratin 15, Keratin 16, Keratin 18 (Phospho-specific Ser33), Keratin 18, Keratin 19, Keratin 20, Keratin 5 / 6 / 18, Keratin 5 / 8, Keratin 6, Keratin 8 (phospho-specific Ser431), Keratin 8 (phospho-specific Ser73), Keratin 8 / 18, Keratin 8, Keratin High Molecular Weight, Keratin Low Molecular Weight, Keratin Multi, Keratin Pan, MAP 2a,b (Microtubule-Associated Protein), MAP 2a,b,c (Microtubule-Associated Protein), MyoD1, Myosin Smooth Muscle Heavy Chain, 3-Oct, Synaptophysin, tau, Transforming Growth Factor alpha (TGFalpha), Vimentin.

 

 T-cell Markers Diferentiation: B7-H2 (ICOS ligand), CD1, CD100 / Leukocyte Semaphorin, CD137 (4-1BB), CD2 (LFA-2), CD231 (TALLA), CD25 / IL-2Ralpha, CD27, CD3 (Early T Cell Marker), CD3zeta / T Cell Receptor zeta, CD4, CD43 (T-Cell Marker), CD7, CD8, CD84, CDw60 (9-O-acetyl GD3), Perforin.

 

G Proteins & G-protein coupled receptors: ADP-ribosylation Factor (ARF) 6, Bonzo / STRL33 / TYMSTR, CXCR4 / Fusin / LESTR / HUMSTR, Gα0 G-Protein, GnRH Receptor / LH-RH Receptor. Hormones: ADP-ribosylation Factor (ARF) 6, Bonzo / STRL33 / TYMSTR, CXCR4 / Fusin / LESTR / HUMSTR, Gα0 G-Protein, GnRH Receptor / LH-RH Receptor, Adrenocorticotrophic Hormone (ACTH), Androgen Receptor, Calcitonin, Cholecystokinin (26-33) (CCK-8), Chorionic Gonadotropin beta, Human (HCG), Estradiol, Estriol, Estrogen Receptor, Follicle Stimulating Hormone (FSH), Follicle Stimulating Hormone-Beta (FSH-Beta), Gastrin 1, Glucagon, Human Growth Hormone (hGH), Inhibin alpha, Insulin, Leukotriene C4 / D4 / E4, Medroxyprogesterone Acetate (MPA), Parathyroid Hormone Receptor (PTH-Receptor) Type 1, Parathyroid Hormone(PTH), Placental Lactogen (hPL), PPAR gamma, Progesterone, Prolactin, Prolactin Receptor, Retinoic Acid Receptor, Beta (hRARBeta), Retinoid X Receptor, gamma Glutamyl Transferase（gGT）, Retinol Binding Protein (RBP), Somatostatin Receptor-1, Testosterone, Thyroid Stimulating Hormone (TSH).

 

Ion Channels: Cystic Fibrosis Transmembrane Regulator (CFTR), GABA α Receptor 1, Human Plasma Membrane Ca2+ Pump (hPMCA), Human Sodium Iodide Symporter (hNIS).

 

Neuroscience: Amyloid A Component, Amyloid A4 , Amyloid Beta, Amyloid A4 Protein Precursor (APP), Apolipoprotein D, Axonal Growth Cones, Calmodulin, CD13 / Aminopeptidase-N, CD56 / NCAM-1, CD90 / Thy-1, Cholecystokinin (26-33) (CCK-8), Chromogranin A, CNPase (Oligodendrocyte & Schwann Cell Marker), CREB, Fascin, GABAA Receptor alpha1, GAD65 (Glutamate Decarboxylase 65), Gastrin 1, GFAP (Glial Fibrillary Acidic Protein), GFAP (Glial Fibrillary Acidic Protein), GluR1, GluR2/3, GluR4, Glycogen Synthase Kinase 3Beta (GSK3Beta), GSK-3, IGF-1 R (alpha-Subunit), IGF-I, IGF-I, MAP 2a,b (Microtubule-Associated Protein), MAP 2a,b,c (Microtubule-Associated Protein), mGluR1 / Grm1 / Metabotropic glutamate receptor 1, mGluR5, Myelin / Oligodendrocyte, Neurofilament (140-160kDa), Neurofilament (200kDa), Neurofilament (68kDa), Neuron Specific Enolase / NSE, Neurotrophin Receptor /NGF-Receptor (p75NGFR), Nitric Oxide Synthase, brain (bNOS), Nitric Oxide Synthase, endothelial (eNOS), Nitric Oxide Synthase, inducible (iNOS), Nitric Oxide Synthase, universal (u-NOS), Notch, p35nck5a, Pan Synuclein, Parkin, PCTAIRE2, PMP-22 (Peripheral Myelin Protein), Presenilin 1 (PS-1), Prostate Apoptosis Response-4, Protein Gene Product 9.5, Ret Oncoprotein, S100 Protein, S100A4, Synaptophysin, Synuclein, Synuclein Beta, tau, Transglutaminase II, Urocortin.

 

6-Histidine (Epitope Tagging), Beta-Galactosidase, Biotin, Bromodeoxyuridine (BrdU), Bromodeoxyuridine (BrdU), Fluorescein Isothiocyanate (FITC), GFP (Green Fluorescent Protein), GST (Epitope Tagging), HA (Hemagglutinin), Luciferase, Maltose Binding Protein.

 

 

 

 

 

上海康成生物工程有限公司

地址：上海市虹漕路421号63号楼2楼
全国免费热线：400-886-5058；800-820-5058
电话：021-64451989  
传真：021-64452021
网址：www.kangchen.com.cn