HHLA2,HHLA2蛋白抗体

产品编号HZ-8419R
英文名称HHLA2
中文名称HHLA2蛋白抗体
别 名Human endogenous retrovirus H long terminal repeat associating protein 2; HERV H LTR associating 2; HERV-H LTR-associating protein 2; HHLA 2; HHLA2; HHLA2_HUMAN; Human endogenous retrovirus-H long terminal repeat-associating protein 2.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human,
HHLA2,HHLA2蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量45kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human HHLA2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
HHLA2,HHLA2蛋白抗体PubMedPubMed
产品介绍background:
Human endogenous retroviruses (HERVs) are repetitive elements that are derived from ancient germline retroviral infections. Due to their ability to move and insert next to certain genes and alter expression patterns, HERVs have been linked to several chronic diseases such as nervous systemic diseases, cancer, autoimmune and connective tissue diseases. The HERV-H family is the most abundant HERV family and has been implicated in the expression of a variety of adjacent genes. Proteins belonging to the HERV-H family are divided into one major and two minor groups based on sequence divergence. As a member of the HERV-H family, HHLA2 (HERV-H LTR-associating protein 2) is a 414 amino acid single-pass type I membrane protein that contains one Ig-like C1-type (immunoglobulin-like) domain and two Ig-like V-type (immunoglobulin-like) domains. HHLA2 is primarily expressed in kidney, lung and intestinal tissues.

Subcellular Location:
Membrane; Single-pass type I membrane protein (By similarity).

Tissue Specificity:
Expressed in colon, small intestine, lung and kidney.

Similarity:
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Database links:
UniProtKB/Swiss-Prot: Q9UM44.1

HHLA2,HHLA2蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-HHLA2 Polyclonal Antibody, Unconjugated(HZ-8419R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining