CHRNA9,烟碱型乙酰胆碱受体α9/AChRα9抗体

产品编号HZ-12113R
英文名称CHRNA9
中文名称烟碱型乙酰胆碱受体α9/AChRα9抗体
别 名Cholinergic receptor nicotinic alpha 9; Cholinergic receptor nicotinic alpha polypeptide 9; HSA243342; MGC142109; MGC142135; NACHR alpha 9; NACHRA9; Neuronal acetylcholine receptor protein subunit alpha 9 precursor; ACHA9_HUMAN.
说 明 书0.1ml 0.2ml
研究领域神经生物学 通道蛋白 细胞膜受体 淋巴细胞 t-淋巴细胞 b-淋巴细胞
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
CHRNA9,烟碱型乙酰胆碱受体α9/AChRα9抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量52kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CHRNA9
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CHRNA9,烟碱型乙酰胆碱受体α9/AChRα9抗体PubMedPubMed
产品介绍background:
Members of the ligand-gated ion channel receptor family are characterized by their fast transmitting response to neurotransmitters. Two important members of this family are the nicotinic acetylcholine and glutamate receptors, both of which are composed of five homologous subunits forming a transmembrane aqueous pore. These transmembrane receptors change conformation in response to their cognate neurotransmitter. Nicotinic acetylcholine receptors (AChRs) are found at the postsynaptic membrane of the neuromuscular junction and bind acetylcholine molecules, allowing ions to move through the pore. AChR alpha 9 is the only AChR found in cochlear hair cells. In adult rat cochlear outer hair cells (OHCs), AChR alpha 9 is expressed primarily in basal regions, where it is a component of the cholinergic receptor, while in inner hair cells (IHCs), it is expressed primarily in apical regions. The alpha 9 subunit mediates efferent synaptic transmission between cholinergic olivocochlear fibers and OHCs. One of the main functions of the AChR alpha 9 channel is to provide a pathway for calcium ion influx. AChR alpha 9 may also influence the arrival of efferent axons.

Function:
CHRNA9 is a member of the ligand gated ionic channel family and nicotinic acetylcholine receptor superfamily. CHRNA9 forms homo- or hetero-oligomeric divalent cation channels. It is involved in cochlea hair cell development and is also expressed in the outer hair cells (OHCs) of the adult cochlea. The protein is additionally expressed in keratinocytes, the pituitary gland, B-cells and T-cells.

Subunit:
Can form homo- or hetero-oligomeric channels in conjunction with CHRNA10. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 hetero-oligomers.

CHRNA9,烟碱型乙酰胆碱受体α9/AChRα9抗体Subcellular Location:
Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein (Probable). Cell membrane; Multi-pass membrane protein (Probable).

Tissue Specificity:
Expressed in cochlea, keratinocytes, pituitary gland, B-cells and T-cells.

Similarity:
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-9/CHRNA9 sub-subfamily.

Gene ID:
55584

Database links:
Entrez Gene: 55584 Human
Entrez Gene: 231252 Mouse
Entrez Gene: 65024 Rat
Omim: 605116 Human
SwissProt: Q9UGM1 Human
SwissProt: P43144 Rat
Unigene: 272278 Human
Unigene: 140015 Mouse
Unigene: 54463 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CHRNA9 Polyclonal Antibody, Unconjugated(HZ-12113R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CHRNA9 Polyclonal Antibody, Unconjugated(HZ-12113R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining