PCR Array

囊性纤维化PCR芯片可用于分析在囊性纤维化（CF）差异表达或与囊性纤维化跨膜电导调节（CFTR）相互作用的84个关键基因的表达。CFTR的突变会引起消化系统和呼吸系统的失调，CF患者会有不同程度的支气管粘液增厚，以及慢性肺炎导致的肺纤维化和肺功能衰竭。目前的研究集中在患者逐渐恶化的CF肺部病变，通过治疗可以延长CF患者40年的平均寿命，但导致疾病及其症状的分子机制尚未发掘。这一芯片包含了离子转运蛋白基因、参与免疫或炎症反应的基因、可能与CFTR功能有关的基因。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测CFTR分子机制相关基因的表达。
Modifier Genes: ADIPOR2, ADRB2, EDNRA, GCLC, GSTM1 (MGST1), IFRD1, IL8, MBL2, MSRA, NOS3 (eNOS), SCNN1B, SCNN1G, SERPINA1, TCF7L2, TGFB1, TNF, TNFRSF1A. Genes Interacting with CFTR: AHSA1, CANX, CFTR, DNAJA1, DNAJC5, EZR, GOPC, HSP90AA1, HSPA4 (HSP70), HSPA8, NME1 (NM23A), PDZK1, PPP2R4, PRKAA1, PRKAA2, SLC26A3, SLC9A3R1, SLC9A3R2, SNAP23, STX1A, STX8, TGFB1, TJP1, VCP. Neutrophil Chemotaxis: CCL2 (MCP1), CXCR2 (IL8RB), EDN1, EDNRA, IL1B, IL8, ITGB2. Inflammatory Response: ACE, ADRB2, ALOX12B, CCL2 (MCP1), CXCL1, CXCL2, DEFB1, EDNRA, IL10, IL1B, IL6, IL8, ITGB2, MBL2, NFKB1, PLA2G5, PTGS2 (COX2), S100A8, SERPINA1, TGFB1, TLR2, TLR4, TLR5, TNF, TNFRSF11A (RANK), TNFRSF1A. Immune Response: Innate: CCL2 (MCP1), CLU, DEFB1, ICAM1, LCN2 (NGAL), MAPK1 (ERK2), MBL2, NFKB1, NFKBIA (I?Ba), PRKCE, TGFB1, TLR2, TLR4, TLR5, TNFSF10 (TRAIL). Adaptive: CLU, FAS (TNFRSF6), ICAM1, IL10, IL1B, IL7R, MBL2, TGFB1, TLR4, TNF, TNFRSF11A (RANK). Unfolded Protein Response: CALR, DNAJA1, HSP90AA1, HSPA1A, HSPA4 (HSP70), HSPA8, HSPH1 (HSP105), VCP. Ion Binding & Transport: ADK, KCNE1, NR4A2 (NUR77), SCNN1B, SCNN1G, SLC9A3R1. Oxidative Stress: CLU, DUSP1 (PTPN16), GCLC, IL6, LCN2 (NGAL), MBL2, MSRA, NOS3 (eNOS), PTGS2 (COX2), SFTPB. Other Genes: EPSTI1, IGFBP5, ITGA2, KIT (CD117), MET, PRTN3, SLPI.