磷酸化视网膜母细胞瘤相关蛋白p130抗体
产品名称： 磷酸化视网膜母细胞瘤相关蛋白p130抗体
英文名称： phospho-Rb2 p130 (Ser952)
产品编号： hz-17585R
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
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Rabbit Anti-phospho-Rb2 p130 (Ser952) antibody
| 产品编号 | hz-17585R |
| 英文名称 | phospho-Rb2 p130 (Ser952) |
| 中文名称 | 磷酸化视网膜母细胞瘤相关蛋白p130抗体 |
| 别 名 | Rb2 p130 (phospho S952); p-Rb2 p130 (phospho S952); p130; PRB2; Rb2; RBL2; RBR2; Retinoblastoma like 2; Retinoblastoma like protein 2. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 128kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KKLH conjugated synthesised phosphopeptide derived from human Rb2 p130 around the phosphorylation site of Ser952:QN(p-S)PT |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: p130 is related both in structure and function to the retinoblastoma tumor suppressor protein and p107 (collectively known as pocket proteins) and is known to regulate the activity of E2F transcription factors. E2F transcription factors regulate the expression of a number of genes important in cell proliferation, particularly those involved in the progression through G1 and into the S phase of the cell cycle. Binding of p130 converts E2F transcription factors from transcriptional activators to transcriptional repressors. Function: Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer. Subcellular Location: Nuclear SWISS: Q08999 Gene ID: 5934 Database links: Entrez Gene: 5934 Human Entrez Gene: 5934 Human Entrez Gene: 19651 Mouse Omim: 180203 Human SwissProt: Q08999 Human SwissProt: 235580 Mouse SwissProt: Q64700 Mouse Unigene: 283604 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
 Sample: A549 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-Rb2 p130 (Ser952) (bs-17585R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 128 kD Observed band size: 128 kD |