GTF2F1抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
GTF2F1抗体

GTF2F1抗体

商家询价

产品名称： GTF2F1抗体

英文名称： GTF2F1Antibody

产品编号： XY10093-2

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 GTF2F1抗体

Applications
In-house applications ELISA, WB, IP     Application not tested? Please see our Guarantee.
Species specificity Human,Mouse,Rat; other species not tested.
Images
Prev1NextView more validation data
  • 10093-2-AP;K-562 cellK-562 cells were subjected to SDS PAGE followed by western blot with 10093-2-AP(GTF2F1 antibody) at dilution of 1:1000
  • 10093-2-AP;K-562 cellsIP Result of anti-GTF2F1 (IP:10093-2-AP, 4ug; Detection:10093-2-AP 1:300) with K-562 cells lysate 4000ug.
Positive WB detected in K-562 cells
Positive IP detected in K-562 cells
Recommended dilution WB : 1:500-1:5000
IP : 1:200-1:1000
 GTF2F1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen GTF2F1 fusion protein ag0133 Full name General transcription factor IIF, polypeptide 1, 74kDa
Calculated molecular
weight		 58 kDa Observed molecular 
weight		 74 kDa
GenBank accession 
number		 BC000120 Gene ID (NCBI) 2962
Gene symbol GTF2F1 Synonyms BTF4, GTF2F1, RAP74, TF2F1, TFIIF, TFIIF alpha
Background GTF2F1抗体

In eukaryotic systems, the initiation of gene transcription involves the ordered assembly of a multiprotein complex on proximal promoter elements, consisting of RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, B, D, E, F and H; and sequence specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, acting at an intermediate stage in initiation complex formation, binds directly to RNA polymerase II in solution and decrease the affinity of RNA polymerase II for nonspecific DNA. In addition, TFIIF stimulates transcription elongation by RNA polymerase II. GTF2F1抗体

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