FITC标记的去整合素样金属蛋白酶2抗体
产品名称： FITC标记的去整合素样金属蛋白酶2抗体
英文名称： Anti-ADAM2/FITC
产品编号： HZ-20815R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-ADAM2/FITC Conjugated antibody
FITC标记的去整合素样金属蛋白酶2抗体
| 英文名称 | Anti-ADAM2/FITC |
| 中文名称 | FITC标记的去整合素样金属蛋白酶2抗体 |
| 别 名 | A disintegrin and metalloproteinase domain 2; ADAM 2; ADAM metallopeptidase domain 2; Adam2; ADAM2_MOUSE; AI323749; Cancer/testis antigen 15; CRYN1; CRYN2; CT15; Disintegrin and metalloproteinase domain-containing protein 2; Fertilin beta; Fertilin subunit beta; Ftnb; PH 30; PH 30b; PH-30; PH30; Ph30 beta; PH30-beta. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 激酶和磷酸酶 细胞骨架 细胞外基质 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 63kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 2mg/1ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse ADAM2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: ADAM-2, also known as PH-30?and Fertilin-? was first described as a sperm-egg fusion protein from guinea pig. A member of the metalloproteinase family containing disintigrin-like domains (ADAMs), the function of ADAM-2 is still poorly understood. Unlike ADAM-1, ADAM-2 does not contain the canonical HExxHxxxxxH zinc metalloproteinase motif, and is not thought to be proteolytically active. Like the other ADAMs, ADAM-2 domain structure consists of a signal sequence followed by a propeptide domain, a metalloproteinase domain, a disintegrin domain cysteine-rich domain, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. Three isoforms of ADAM-2 are reported to date, which differ in the beginning of the metalloproteinase domain and cysteine-rich domain. The sequences are coded from intronless genes. The longest ADAM-2 message encodes a protein of 735 amino acids, with a predicted mass of 82.5 kDa and a pI of 5.73. The 716 amino acid form of ADAM-2 has a deletion in the start of the metalloproteinase domain, relative to the longer form, and has a predicted mass of 80.2 kDa and pI of 5.75. The shorter form of 579 amino acids shared the deletion in the MP domain, and also has a deletion in the cystein-rich domain. Localized on the surface of sperm, the ADAM-2 isoforms are thought to form a heterodimer with ADAM-1 (fertilin-a), and facilitate sperm-egg fusion, although there is some controversy about the precise actions the proteins play. Integrin a6? on the egg surface is thought to dock with a QDECD motif in the disintegrin domain of ADAM-2, and there is some speculation that ADAM-1/ADAM-2 heterodimewr initiated ADAM-3 production on the cell surface. Function: Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein. Subunit: Heterodimer with ADAM1/fertilin subunit alpha. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed specifically in testis. Post-translational modifications: The prodomain and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa. Similarity: Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain. Database links: Entrez Gene: 2515 Human Omim: 601533 Human SwissProt: Q99965 Human Unigene: 177959 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
ADAM-2，又称PH-30？施肥呢？首次被描述为豚鼠精子卵融合蛋白。作为含有去内膜素样结构域（ADAM）的金属蛋白酶家族的成员，ADAM-2的功能仍不清楚。与ADAM-1不同，ADAM-2不包含典型的HExxHxxxxH锌金属蛋白酶基序，并且被认为不具有蛋白水解活性。与其他ADAM一样，ADAM-2结构域由信号序列组成，接着是前肽结构域、金属蛋白酶结构域、去整合素结构域、富含半胱氨酸结构域、EGF结构域、跨膜结构域和细胞质结构域。ADAM-2有三种亚型，它们在金属蛋白酶结构域和富含半胱氨酸结构域的起始部分有所不同。序列由无内含子基因编码。最长的ADAM-2信息编码735个氨基酸的蛋白质，预测质量为82.5kDa，pI为5.73。ADAM-2的716氨基酸形式在金属蛋白酶结构域开始时相对于较长形式有缺失，并且预测质量为80.2kDa，pI为5.75。短序列的579个氨基酸在MP结构域共享缺失，在富含半胱氨酸结构域也有缺失。ADAM-2异构体定位于精子表面，被认为与ADAM-1（受精-a）形成异二聚体，并促进精子-卵子融合，尽管对于这些蛋白质发挥的精确作用存在一些争议。整合素A6？在鸡蛋表面被认为与ADAM-2的解整合素结构域中的QDECD基序对接，并且有推测ADAM-1/ADAM-2异源二聚体在细胞表面启动了ADAM-3的产生。