Cyclin E1,周期素E1抗体

产品编号HZ-8929R
英文名称Cyclin E1
中文名称周期素E1抗体
别 名CCNE 1; CCNE; CCNE1; Cyclin E1 antibody Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; CCNE1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 神经生物学 细胞凋亡 细胞周期蛋白 新陈代谢
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human,
Cyclin E1,周期素E1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:30-300 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量47kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Cyclin E
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Cyclin E1,周期素E1抗体PubMedPubMed
产品介绍background:
Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Function:
Essential for the control of the cell cycle at the G1/S (start) transition.

Subunit:
Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation.

Subcellular Location:
Nucleus.

Tissue Specificity:
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

Post-translational modifications:
Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.

Cyclin E1,周期素E1抗体Similarity:
Belongs to the cyclin family. Cyclin E subfamily.

Gene ID:
898

Database links:
Entrez Gene: 898 Human
Entrez Gene: 12447 Mouse
Entrez Gene: 25729 Rat
Omim: 123837 Human
SwissProt: P24864 Human
SwissProt: Q61457 Mouse
SwissProt: P39949 Rat
Unigene: 244723 Human
Unigene: 16110 Mouse
Unigene: 15455 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

细胞周期素E是调控细胞G-1→S期转变的关键因素。由于在多种肿瘤中的不适当表达,细胞周期素E现在已明确为原癌基因。