北京智杰方远代理台湾旭基――病毒核酸纯化试剂盒VR100现货促销

提供全套检疫证明、报关单、COA、产品生物安全性保证书等文件

www.biozj.com，  QQ：523539965，  电话：010-82897201， E－Mail:15810200961@163.com

Virus DNA/RNA Extraction Kit II VR050/VR100/VR300

The Viral Nucleic Acid Extraction Kit II was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. The efficient glass fiber spin column system is optimized for nucleic acid purification from a wide variety of both DNA and RNA viruses such as HBV, CMV, HCV, HIV, and HTLV. 10¹-10⁹ copies of viral DNA/RNA can be purified from up to 200 µl samples within 20 minutes. The purified viral DNA/RNA can be used directly in qPCR and qRT-PCR assays. 

Advantages (Cat. # VR050, VR100, VR300)

• High Sensitivity: virus RNA/DNA can be successfully extrac　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　ted and detected from as low as 10E1 copy number!
• Purify virus DNA or virus RNA in 20 minutes!
• Sample Volume: up to 200 µl samples of plasma, serum, body fluids, supernatant of viral cell cultures
• Spin Columns: glass fiber membrane optimized for virus DNA and virus RNA purification
• Individually packaged virus spin columns and collection tubes, certified RNase and DNase-free
• Elution Volume: 50 µl
• Storage: dry at room temperature (15-25ºC)

Applications

RT-PCR/PCR, qPCR, qRT-PCR, Real-time PCR, Real-time RT-PCR, Automated Fluorescent DNA Sequencing, Next Generation Sequencing (NGS) 

Components

• VB Lysis Buffer
• AD Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• VB Columns
• 2 ml Collection Tubes

Quality Control

The quality of Viral Nucleic Acid Extraction Kit II is tested on a lot-to-lot basis according to Geneaids ISO-certified quality management system by isolating viral DNA/RNA from a 200 µl serum sample.

Viral Nucleic Acid Extraction Kit II Functional Test Data

Figure 1. Virus RNA was purified from 10E1-10E4 copy number of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) using the Geneaid Virus DNA/RNA Kit II (3 replications of each copy number). The purified RNA was eluted with 30 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 3 μl of synthesized cDNA as template, primers (designed to amplify the T4 region on the RNA2 segment), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that virus RNA can be successfully extracted and detected from as low as 10E1 copy number of RGNNV. The average cycle threshold (Ct): 10E4 = 23.88, 10E3 = 27.72, 10E2 = 31.22, 10E1 = 34.62. The low Ct values indicate a high number of target nucleic acid in the sample.

Publications

Journal Article 1 

Journal Article 2

Journal Article 3 

Journal Article 4

Journal Article 5

Related Virus DNA/RNA Purification Products

病毒基因组DNA/RNA 提取试剂盒

仅供科研使用

产品编号

VR050, VR100, VR300

完整说明书(英文)下载

如果您是第一次使用本产品或对本产品的操作步骤不熟悉,请您经由扫描QR code下载并详阅完整说明书 

1. 处理材料

取200 μl样本（例如血浆,血清,淋巴液或经病毒感染的细胞培养液）转移到1.5 ml微量离心管中.

注意：样本不足200 μl可加缓冲液PBS补足.

加入400 μl缓冲液VB Lysis,使用涡旋振荡器振荡混匀,室温放置10分钟. 

2. 吸附

加入450 μl缓冲液AD（使用前请先检查是否已加入无水乙醇),充分摇动混匀. 将吸附柱VB放入2 ml收

集管中. 将600 μl所得溶液加入吸附柱VB中,以14-16,000×g离心1TUNEology 波长可调检测卡盒-->