兔烯醇化酶(ENO3)多克隆抗体
Polyclonal Antibody to Enolase 3 (ENO3)
[PRODUCT INFORMATION]
Immunogen: MSE, Rabbit
Clonality: Polyclonal
Host: Cavia
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant MSE (Met3~Lys434) expressed in E.coli. 
Sequence: The target protein is fused with N-terminal His-Tag and its sequenceis listed below.
MGHHHHHHSG SEF-MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGASTGIYEALELR DGDKSRYLGK GVLKAVEHIN KTLGPALLEK KLSVVDQEKVDKFMIELDGT
ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN HDLVLPVPAF NVINGGSHAGNKLAMQEFMI LPVGASSFRE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILENNEALELLKTA IQAAGYPDKV VIGMDVAASE FHRNGKYDLD FKSPDDPARH ITGQKLGELYKSFIKNYPVV SIEDPFDQDD WATWTSFLSG VDIQIVGDDL TVTNPKRIAQ AVEKKACNCLLLKVNQIGSV TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCRSERLAKYNQL MRIEEALGDK AVFAGRKFRN PKAK
[ ANTIBODY SPECIFITY ]
The antibody is a cavia polyclonal antibody raised against MSE. It has beenselected for its ability to recognize MSE in immunohistochemical staining andwestern blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[CONTENTS ] 
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.
[QUALITY CONTROL ] 
Content:The quality control contains recombinant MSE (Met3~Lys434) disposedin loading buffer
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%
[STORAGE ] 
Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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