FITC标记的DNA糖基化酶FPG2抗体
产品名称： FITC标记的DNA糖基化酶FPG2抗体
英文名称： Anti-NEI3/FITC
产品编号： HZ-19191R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-NEI3/FITC Conjugated antibody
FITC标记的DNA糖基化酶FPG2抗体
| 英文名称 | Anti-NEI3/FITC |
| 中文名称 | FITC标记的DNA糖基化酶FPG2抗体 |
| 别 名 | 123; AI449477; BC034753; C85903; DNA glycosylase FPG2; DNA glycosylase hFPG2; DNA glycosylase/AP lyase Neil3; EC 3.2.2.-; EC 4.2.99.18; Endonuclease 8-like 3; Endonuclease VIII-like 3; FGP2; FLJ10858; FPG2; hFPG2; hNEI3; MGC27861; MGC36916; Nei endonuclease VIII-like 3 (E. coli); nei endonuclease VIII-like 3; Nei like 3 (E. coli); Nei-like protein 3; NEIL3; NEIL3_HUMAN; OTTHUMP00000218810; RGD1310562. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Horse, Rabbit, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 68kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NEI3 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008] Function: Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single-stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected. Subcellular Location: Nucleus. Tissue Specificity: Detected in thymus and testis. Expressed at higher levels in tumor tissue than in the corresponding normal tissues except for pancreas and testis where expression is higher in normal than tumor tissue. Similarity: Belongs to the FPG family. Contains 1 FPG-type zinc finger. Contains 1 RanBP2-type zinc finger. Database links: Entrez Gene: 55247 Human Entrez Gene: 234258 Mouse Entrez Gene: 290729 Rat Omim: 608934 Human SwissProt: Q8TAT5 Human SwissProt: Q8K203 Mouse SwissProt: D3ZKJ8 Rat Unigene: 405467 Human Unigene: 281749 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
NeIL3属于一类与细菌FPG/NEI家族同源的DNA糖基化酶。这些糖基化酶通过裂解被活性氧物种破坏的碱基并通过相关的裂解酶反应引入DNA链断裂，开始了碱基切除修复的第一步(Bandaru等人，2002[PubMed 12509226])。