AKAP7抗体
Applications
| In-house applications |
ELISA, IHC, IF, WB, IP Application not tested? Please see our Guarantee. |
| Published applications |
WB |
Publications |
2 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human, mouse |
|
Images
Prev12Next
 human brain tissue were subjected to SDS PAGE followed by western blot with 12591-1-AP(AKAP7 antibody) at dilution of 1:500 Immunofluorescent analysis of HepG2 cells, using AKAP7 antibody 12591-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). IP Result of anti-AKAP7 (IP:12591-1-AP, 4ug; Detection:12591-1-AP 1:300) with mouse brain tissue lysate 4000ug. Immunohistochemistry of paraffin-embedded human brain tissue slide using 12591-1-AP( AKAP7 Antibody) at dilution of 1:50 (under 10x lens) Immunohistochemistry of paraffin-embedded human brain tissue slide using 12591-1-AP( AKAP7 Antibody) at dilution of 1:50 (under 40x lens)
|
| Positive WB detected in |
Human brain tissue, human heart tissue |
| Positive IP detected in |
Mouse brain tissue |
| Positive IHC detected in |
Human brain tissue, human heart tissue |
| Positive IF detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:200-1:2000
IP : 1:200-1:1000
IHC : 1:20-1:200
IF : 1:10-1:100 |
AKAP7抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
AKAP7 fusion protein ag3297 |
Full name |
A kinase (PRKA) anchor protein 7 |
Calculated molecular
weight |
81aa,9 kDa |
Observed molecular
weight |
11 kDa,18 kDa,37 kDa |
GenBank accession
number |
BC016927 |
Gene ID (NCBI) |
9465 |
| Gene symbol |
AKAP7 |
Synonyms |
A kinase anchor protein 18 kDa, AKAP 18, AKAP 7 isoforms alpha and beta, AKAP18, AKAP7 |
Background AKAP7抗体
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AKAP7, also named as AKAP15 and AKAP18, localize PKA in complexes with CaV1.2 and PLN, respectively. AKAP7 targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. AKAP7 has short isoform AKAP7 alpha and AKAP7 beta with MW 15-18 kDa; long isoform AKAP7 gamma with MW 37-42 kDa. AKAP7alpha was highly expressed only in brain and weakly in lung lysates from WT animals. This antibody can recognize all the isoforms of AKAP7. AKAP7抗体
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