CASPASE 3抗体
Applications
| In-house applications |
ELISA, IHC, WB, IF, IP Application not tested? Please see our Guarantee. |
| Published applications |
IF, IHC, WB |
Publications |
73 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Baby Hamster Syrian, human, mouse, rat |
|
Images
Prev12Next
 Jurkat cells were subjected to SDS PAGE followed by western blot with 19677-1-AP( Caspase 3 Antibody) at dilution of 1:600 Immunohistochemical of paraffin-embedded human kidney using 19677-1-AP(CASP3 antibody) at dilution of 1:50 (under 10x lens) Immunohistochemical of paraffin-embedded human kidney using 19677-1-AP(CASP3 antibody) at dilution of 1:50 (under 40x lens) IP Result of anti-Caspase 3 (IP:19677-1-AP, 4ug; Detection:19677-1-AP 1:500) with NIH/3T3 cells lysate 2000ug. Immunofluorescent analysis of ( -20℃ Ethanol ) fixed NIH/3T3 cells using 19677-1-AP( Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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| Positive WB detected in |
Jurkat cells, HeLa cells, mouse spleen tissue |
| Positive IP detected in |
NIH/3T3 cells |
| Positive IHC detected in |
Human kidney tissue, human spleen tissue |
| Positive IF detected in |
NIH/3T3 cells |
| Recommended dilution |
WB : 1:200-1:2000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200 |
CASPASE 3抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information CASPASE 3抗体
| Immunogen |
Peptide |
Full name |
Caspase 3, apoptosis-related cysteine peptidase |
Calculated molecular
weight |
32 kDa |
Observed molecular
weight |
32-35 kDa |
GenBank accession
number |
NM_004346 |
Gene ID (NCBI) |
836 |
| Gene symbol |
CASP3 |
Synonyms |
Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1 |
Background
|
Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Caspase 3 is involved in the cleavage of huntingtin. The antibody can recognize p17 and p32 of Caspase 3. CASPASE 3抗体
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