KAMIYA BIOMEDICAL COMPANY
Rev. 032906
PRODUCT DATA SHEET
KAMIYA BIOMEDICAL COMPANY, Seattle, WA USA Tel: (206) 575-8068 • Fax: (206) 575-8094
www.kamiyabiomedical.com techsupport@kamiyabiomedical.com
Product: Anti-Daxx (C-terminus), polyclonal
Cat. No.: PC-015 (100 μg)
Background:
Apoptosis, or programmed cell death, occurs
during normal cellular differentiation and
development of multicellular organisms.
Apoptosis is induced by certain cytokines
including TNF and Fas ligand of the TNF family
through their death domain containing receptors,
TNFR1 and Fas. Cell death signals are
transduced by death domain (DD)- containing
adapter molecules and members of the
ICE/CED-3 protease family. A novel DDcontaining
molecule was cloned from mouse,
human and monkey and designated Daxx. Daxx
binds specifically to the Fas death domain and
enhances Fas induced apoptosis and activates
the Jun N-terminal kinase (JNK) pathway. Daxx
is widely expressed in fetal and adult human and
mouse tissues indicating its important function in
Fas signaling pathways.
Species Reactivity:
Human, mouse and rat. Other speicies not
tested.
Ig Isotype:
Rabbit IgG
Positive Control:
HeLa whole cell lysate can be used as a positive
control.
Immunogen:
A peptide corresponding to amino acids 722-740
at the C-terminus of human Daxx.
Format:
Purified IgG, 100 μg in 200 μL of PBS containing
0.02% sodium azide.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Suggested dilution: 1:500 to
1:1,000.

Immunohistochemistry
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.
Western blot analysis of 50
μg of total cell lysate from
HeLa cells with anti-Daxx
(CT) at 1:1,000 dilution.