Phospho-Ezrin (Tyr353),磷酸化埃兹蛋白抗体

产品编号HZ-3133R
英文名称Phospho-Ezrin (Tyr353)
中文名称磷酸化埃兹蛋白抗体
别 名Ezrin (phospho Y353); Ezrin (phospho Tyr353); p-Ezrin (phospho Y353); Ezrin (phospho Y354); Ezrin (phospho Tyr354); CVIL; CVL; cytovillin 2; Cytovillin; DKFZp762H157; FLJ26216; MGC1584; p81; VIL 2; VIL2; Villin 2; Villin2; EZRI_HUMAN.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域肿瘤 心血管 细胞生物 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Dog, Pig, Cow, Horse, Rabbit,
Phospho-Ezrin (Tyr353),磷酸化埃兹蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量64kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Ezrin around the phosphorylation site of Tyr353
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Phospho-Ezrin (Tyr353),磷酸化埃兹蛋白抗体PubMedPubMed
产品介绍background:
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

Function:
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Subunit:
Interacts with MPP5 and SLC9A3R2. Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B. Interacts (when phosphorylated) with FES/FPS.

Subcellular Location:
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.

Tissue Specificity:
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.

Post-translational modifications:
Phosphorylated by tyrosine-protein kinases.

Phospho-Ezrin (Tyr353),磷酸化埃兹蛋白抗体Similarity:
Contains 1 FERM domain.

Gene ID:
7430

Database links:
Entrez Gene: 7430 Human
Entrez Gene: 281574 Cow
Entrez Gene: 22350 Mouse
Omim: 123900 Human
SwissProt: P31976 Cow
SwissProt: P15311 Human
SwissProt: P26040 Mouse
Unigene: 487027 Human
Unigene: 277812 Mouse

Phospho-Ezrin (Tyr353),磷酸化埃兹蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

信号传导（Signaling Intermediates）
目前研究发现Ezrin蛋白与维持细胞的形状、极性、生长运动以及信号转导方面发挥重要作用，该蛋白与肿瘤的侵袭、转移有关。