核酮糖1,5二磷酸羧化酶/二磷酸核酮糖盐羧化酶大链抗体
产品名称: 核酮糖1,5二磷酸羧化酶/二磷酸核酮糖盐羧化酶大链抗体
英文名称: RuBisCO large subunit
产品编号: hz-6988R
产品价格: null
产品产地: 中国/上海
品牌商标: HZbscience
更新时间: 2023-08-17T10:24:20
使用范围: WB=1:500-1000 ELISA=1:500-1000 IHC-F=1:400-800 IF=1:50-200
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Rabbit Anti-RuBisCO large subunit antibody
产品编号 | hz-6988R |
英文名称 | RuBisCO large subunit |
中文名称 | 核酮糖1,5二磷酸羧化酶/二磷酸核酮糖盐羧化酶大链抗体 |
别 名 | rbcL; RBL_SPIOL; Ribulose bisphosphate carboxylase large chain; RuBisCO-large subunit; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast) [Arabidopsis lyrata]. |
产品类型 | 植物抗体 |
研究领域 | 肿瘤 细胞生物 信号转导 新陈代谢 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Arabidopsis Thaliana, ,Gourd,Lotus,Linden |
产品应用 | WB=1:500-1000 ELISA=1:500-1000 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 52kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from Arabidopsis thaliana Rubisco:151-250/475 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | Function: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. Subunit: Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers. Subcellular Location: Plastid, chloroplast. Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover. The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization. Similarity: Belongs to the RuBisCO large chain family. Type I subfamily. SWISS: P00875 Gene ID: 2715621 Database links: Entrez Gene: 2715621 Spinacia oleracea SwissProt: P00875 Spinacia oleracea Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 |
Sample: Linden Lysate at 40 ug
Primary: Anti-RUBISCO (bs-6988R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52 kD Sample:
Gourd leaf Lysate at 40 ug Lotus leaf Lysate at 40 ug Primary: Anti-RUBISCO (bs-6988R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52 kD |