HMOX1抗体
Applications
| In-house applications |
ELISA, WB, IHC, IP, IF Application not tested? Please see our Guarantee. |
| Published applications |
IHC, WB |
Publications |
20 citations –view here |
| Species specificity |
Human, Mouse, Rat; other species not tested. |
| Published Species |
Human, MOUSE, rat |
|
Images
Prev12Next
 mouse liver tissue were subjected to SDS PAGE followed by western blot with 10701-1-AP(HMOX1 antibody) at dilution of 1:600 IP Result of anti-HMOX1 (IP:10701-1-AP, 3ug; Detection:10701-1-AP 1:1000) with HeLa cells lysate 3000ug. Immunohistochemical of paraffin-embedded human kidney using 10701-1-AP(HMOX1 antibody) at dilution of 1:50 (under 10x lens) Immunohistochemical of paraffin-embedded human kidney using 10701-1-AP(HMOX1 antibody) at dilution of 1:50 (under 40x lens) Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HT-1080 cells using 10701-1-AP( HO-1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
|
| Positive WB detected in |
Mouse liver tissue, HeLa cells, HepG2 cells, HT-1080 cells, rat spleen tissue |
| Positive IP detected in |
HeLa cells |
| Positive IHC detected in |
Human kidney tissue |
| Positive IF detected in |
HT-1080 cells |
| Recommended dilution |
WB : 1:200-1:2000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:20-1:200 |
HMOX1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen InformationHMOX1抗体
| Immunogen |
HMOX1 fusion protein ag1190 |
Full name |
Heme oxygenase (decycling) 1 |
Calculated molecular
weight |
33 kDa |
Observed molecular
weight |
28-33 kDa |
GenBank accession
number |
BC001491 |
Gene ID (NCBI) |
3162 |
| Gene symbol |
HMOX1 |
Synonyms |
BK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HSP32 |
Background
|
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron .It has 3 isoforms:HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed(PMID:10194478 ).Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells(PMID:15451051 )and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin(PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa)(James R. Reed,Pharmacology ,535-568).HMOX1抗体
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