PCP4,脑特异性多肽蛋白19抗体

产品编号HZ-11326R
英文名称PCP4
中文名称脑特异性多肽蛋白19抗体
别 名Brain specific antigen PCP 4; Brain specific polypeptide PEP 19; Brain specific polypeptide PEP19; PEP19; Purkinje cell protein 4; PCP-4; PCP4_HUMAN; PEP-19.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Cow, Sheep,
PCP4,脑特异性多肽蛋白19抗体产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量7kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PCP4
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PCP4,脑特异性多肽蛋白19抗体PubMedPubMed
产品介绍background:
PCP-4 is a calmodulin (CaM) regulatory protein that is highly expressed in neuronal cells. Through its IQ motif, PCP-4 mediates both the calcium-dependent binding properties of CaM and the rates of association and dissociation of calcium from the C-terminal domain of CaM. The IQ motif contains a serine residue which can be phosphorylated by all isoforms of protein kinase C (PKC). PCP-4 is implicated in uterine leiomyomas, as well as in neurodegenerative disorders such as Alzheimer’s disease and Huntington's disease. Additionally, overexpression of PCP-4 is thought to play a role in cerebellar hypoplasia, a key feature of Down syndrome.

Function:
Probable regulator of calmodulin signaling.

Subunit:
Binds to both calcium-free and calcium activated calmodulin, affinity for calcium-bound form is 50-fold greater.

Similarity:
Belongs to the PCP4 family.
Contains 1 IQ domain.

Database links:
Entrez Gene: 5121 Human
Entrez Gene: 18546 Mouse
Entrez Gene: 25510 Rat
Omim: 601629 Human
SwissProt: P48539 Human
SwissProt: P63054 Mouse
SwissProt: P63055 Rat
Unigene: 80296 Human
Unigene: 5023 Mouse
Unigene: 9736 Rat

PCP4,脑特异性多肽蛋白19抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-PCP4 Polyclonal Antibody, Unconjugated(HZ-11326R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining