DAZL,生殖细胞发育相关蛋白DAZL抗体

产品编号HZ-12245R
英文名称DAZL
中文名称生殖细胞发育相关蛋白DAZL抗体
别 名DAZ homolog; DAZ like autosomal; DAZ-like autosomal; deleted in azoospermia-like; DAZH; DAZL 1; DAZL; DAZL_HUMAN; DAZL1; DAZLA; Deleted in azoospermia like 1; Deleted in azoospermia like; Deleted in azoospermia like autosomal; Deleted in azoospermia-like 1; Deleted in azoospermia-like; Germline specific RNA binding protein; MGC26406; Spermatogenesis gene on the Y like autosomal; SPGY like autosomal; SPGY-like-autosomal; SPGYLA; Tpx2.
说 明 书0.1ml 0.2ml
研究领域发育生物学 干细胞 转录调节因子 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep,
DAZL,生殖细胞发育相关蛋白DAZL抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量33kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human DAZL (51-130aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
DAZL,生殖细胞发育相关蛋白DAZL抗体PubMedPubMed
产品介绍background:
The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010].

Function:
RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.

Subunit:
Homodimer and heterodimer. Forms a heterodimer with DAZ. Interacts with BOLL, DAZAP1 and DAZAP2. Interacts with PUM2.

DAZL,生殖细胞发育相关蛋白DAZL抗体Subcellular Location:
Cytoplasm. Nucleus. Predominantly cytoplasmic. Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.

Tissue Specificity:
Testis specific.

Similarity:
Belongs to the RRM DAZ family.
Contains 1 DAZ-like domain.
Contains 1 RRM (RNA recognition motif) domain.

Gene ID:
1618

Database links:
Entrez Gene: 530116 Cow
Entrez Gene: 1618 Human
Entrez Gene: 13164 Mouse
Omim: 601486 Human
SwissProt: Q92904 Human
SwissProt: Q64368 Mouse
Unigene: 63695 Cow
Unigene: 131179 Human
Unigene: 280641 Mouse

DAZL,生殖细胞发育相关蛋白DAZL抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.