干细胞miRNA PCR芯片(Stem Cell miScript miRNA PCR Array)
产品名称： 干细胞miRNA PCR芯片(Stem Cell miScript miRNA PCR Array)
英文名称： Stem Cell miScript miRNA PCR Array
产品编号： yb229
产品价格： 4800
产品产地： null
品牌商标： null
更新时间： null
使用范围： null
- 联系人 : 谷小姐
- 地址 : 上海市闵行区浦江镇联航路1239弄8B号3楼
- 邮编 : 201112
- 所在区域 : 上海
- 电话 : 181****9791 点击查看
- 传真 : 点击查看
- 邮箱 : gulingzhi@yingbio.com
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miScript miRNA PCR Array |
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人类干细胞miRNA的PCR芯片用于研究干细胞感应、维护、分化和识别相关的84 个miRNA的表达。所有的干细胞都能够自我更新和分化成多种细胞类型。多能干细胞能够分化成3胚层的任何类型细胞，而3胚层表达一组对多能性至关重要的miRNA，包括可以提高重编程使分化细胞回到干细胞状态。然而,最近的研究表明, 与胚胎干细胞(ESC)相比,诱导多能干细胞(iPSC)和始发态（或外胚层）干细胞(epiSC)的miRNA表达有差异。在分化过程,多能性标记物的表达水平下降而其他分化关键miRNA的表达增加。附加的miRNA在特定阶段或在特定血统变得更加普遍,导致miRNA对三种不同胚层祖细胞独特的表达模式。这个芯片确定miRNA的相对表达对多能性和分化干细胞重要性。芯片还额外包括代表胚胎干细胞(ESC),诱导多能干细胞(iPSC),外胚层干细胞(epiSC),胚状体的身体细胞,胚层祖细胞的标记。每张芯片含一个对照组使得分析数据时可以用相对定量ΔΔCT方法评估逆转录效率,基因组DNA污染,和PCR效率。利用这张芯片，通过实时定量PCR，可以简易且可靠地检测与干细胞最相关的miRNA的表达。
Pluripotency Markers:miR-302a-3p, miR-302a-5p, miR-302b-3p, miR-302b-5p, miR-302c-3p, miR-302c-5p, miR-302d-3p, miR-367-3p, miR-371a-5p, miR-372-3p, miR-373-3p, miR-373-5p.
Pluripotency Reprogramming Enhancers:miR-302a-3p, miR-302a-5p, miR-302b-3p, miR-302b-5p, miR-302c-3p, miR-302c-5p, miR-302d-3p, miR-367-3p.
Embryonic Stem Cell (ESC) Markers:miR-182-5p, miR-222-3p, miR-517a-3p, miR-518b, miR-518c-3p, miR-520b, miR-520e, miR-520g-3p.
Induced Pluripotent Stem Cell (iPSC) Markers:miR-106b-5p, miR-125b-5p, miR-136-5p, miR-141-3p, miR-142-3p, miR-18a-5p, miR-181b-5p, miR-196a-5p, miR-196b-5p, miR-199b-3p, miR-19a-3p, miR-20a-5p, miR-214-3p, miR-221-3p, miR-24-2-5p, miR-24-3p, miR-25-3p, miR-29a-3p, miR-30e-5p, miR-323a-3p, miR-410-3p, miR-495-3p, miR-517a-3p, miR-518b, miR-518c-3p, miR-92a-3p.
Epiblast or Primed Stem Cell (epiSC) Markers:miR-135a-5p, miR-141-3p, miR-181b-5p, miR-200a-3p, miR-200b-3p, miR-218-5p, miR-375, miR-429-3p, miR-487b-3p, miR-489-3p, miR-520a-3p, miR-539-5p, miR-665, miR-96-5p.
Differentiation Factors:let-7a-5p, let-7b-5p, let-7c-5p, let-7d-5p, let-7e-5p, let-7f-5p, let-7g-5p, let-7i-5p, miR-106b-5p, miR-17-3p, miR-17-5p, miR-200a-3p, miR-200b-3p, miR-200c-3p, miR-20a-5p, miR-93-5p, miR-96-5p.
Embryoid Body Markers:miR-323a-3p, miR-487b-3p, miR-489-3p, miR-539-5p, miR-665.
Germ Layer Differentiation:
Ectodermal Progenitor Cell Markers:miR-134-5p, miR-135b-5p, miR-18a-5p, miR-18b-5p, miR-200a-3p, miR-200b-3p, miR-200c-3p, miR-21-5p, miR-214-3p, miR-323a-3p, miR-376a-3p, miR-424-5p, miR-495-3p, miR-96-5p, miR-98-5p.
Endodermal Progenitor Cell Markers:miR-10a-5p, miR-103a-3p, miR-122-5p, miR-136-5p, miR-141-3p, miR-17-3p, miR-17-5p, miR-192-5p, miR-196a-5p, miR-196b-5p, miR-206, miR-218-5p, miR-224-5p, miR-24-3p, miR-326, miR-335-5p, miR-375, miR-93-5p.
Mesodermal Progenitor Cell Markers:miR-125b-5p, miR-141-3p, miR-145-5p, miR-17-3p, miR-17-5p, miR-18a-5p, miR-18b-5p, miR-202-3p, miR-335-5p, miR-93-5p, miR-98-5p.
Mesenchymal Stem Cells Markers:miR-10a-5p, miR-145-5p, miR-181b-5p, miR-196a-5p, miR-196b-5p, miR-21-5p, miR-214-3p, miR-218-5p, miR-221-3p, miR-222-3p, miR-29a-3p, miR-424-5p, miR-98-5p.