PCR Array

细胞表面标志物PCR芯片可用于分析与细胞表面标志物水平有关的84个关键基因的表达。这些标志物已经应用于以下研究：1）测定白细胞在癌症组织中的浸润程度；2）诊断慢性炎性疾病，如动脉粥样硬化、哮喘、肠炎和关节炎；3）确定T细胞/B细胞系和单克隆；4）测定白细胞数量从而定疾病的阶段，发生、发展或预后；5）分析免疫浸润的原因及其与器官排异反应的关系。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测细胞表面标志物相关基因的表达。
B-cell Surface Markers: Activated B-cells: CD28, CD38, CD69, CD80, CD83, CD86, DPP4, FCER2, IL2RA, TNFRSF8, CD70 (TNFSF7). Mature B-cells: CD19, CD22, CD24, CD37, CD40, CD72, CD74, CD79A, CD79B, CR2, IL1R2, ITGA2, ITGA3, MS4A1, ST6GAL1. Other B-cell Surface Markers: CD1C, CHST10, HLA-A, HLA-DRA, NT5E. T-cell surface markers: Cytotoxic T-cells: CD8A, CD8B. Helper T-cells: CD4. Activated T-cells: ALCAM, CD2, CD38, CD40LG, CD69, CD83, CD96, CTLA4, DPP4, HLA-DRA, IL12RB1, IL2RA, ITGA1, TNFRSF4, TNFRSF8, CD70 (TNFSF7). Other T-cell Surface Markers: CD160, CD28, CD37, CD3D, CD3G, CD247, CD5, CD6, CD7, FAS, KLRB1, KLRD1, NT5E, ST6GAL1. Natural Killer (NK) Cell Surface Markers: CD2, CD244, CD247, CD7, CD96, CHST10, IL12RB1, KLRB1, KLRC1, KLRD1, NCAM1. Monocyte and Macrophage Cell Surface Markers: Activated Macrophages: CD69, ENG, FCER2, IL2RA. Other Monocyte and Macrophage Surface Markers: C5AR1, CD163, CD40, CD63, CD74, CD86, CHST10, CSF1R, DPP4, FCGR1A, HLA-DRA, ICAM2, IL1R2, ITGA1, ITGA2, S100A8, TNFRSF8, CD70 (TNFSF7). Endothelial Cell Surface Markers: ENG, ICAM2, NOS3, PECAM1, SELP, TEK, VCAM1, VWF. Smooth Muscle Cell Surface Markers: MYH10, MYH9, MYOCD. Dendritic Cell Surface Markers: CD1A, CD209, CD40, CD83, CD86, CR2, FCER2. Mast Cell Surface Markers: C5AR1, FCER1A, FCER2, TPSAB1. Fibroblast (Stromal) Surface Markers: ALCAM, COL1A1, COL1A2. Epithelial Cell Surface Markers: CD1D, KRT18, KRT5, KRT8, EPCAM. Adipocyte Surface Markers: RETN.