产品名称

产品编号

规格

Recombinant Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein,His-Avi Tag

93021ES25

25 μg

93021ES60

100 μg

93021ES76

500 μg

蛋白别名（Synonyms）

sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G;

表达系统及表达区间（Source）

Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.[Accession | P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL]

分子量（Molecular Weight）

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on SDS-PAGE result.

内毒素（Endotoxin）

< 1EU per μg by the LAL method.

活性（Activity）

Blocking Data: Serial dilutions of Anti-LILRB2 Antibody were added into Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag : Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.11μg/ml.

ELISA Data: Immobilized Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.

SPR Data: Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer with an affinity constant of 4.62 nM as determined in SPR assay .Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5μM as determined in a SPR assay .

纯度（Purity）

> 95% as determined by SDS-PAGE and by HPLC.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.