人凋亡诱导因子(AIF)多克隆抗体
Polyclonal Antibody to Apoptosis Inducing Factor (AIF)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific affinity chromatography followed by Protein A affinity
chromatography
Traits: Liquid
Concentration: 500µg/mL
UOM: 1
Cross Reactivity: Porcine; Bovine
Applications: WB,IHC,ICC/IF
[ IMMUNOGEN ]
Immunogen:Recombinant AIF (Gly103~Lys322 expressed in E.coli
[ APPLICATIONS ]
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 3-30µg/mL;
Immunocytochemistry: 3-30µg/mL;
Optimal working dilutions must be determined by end user.
[ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol.
[ STORAGE AND STABILITY ] 
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ IDENTIFICATION ]


Western Blot; Sample: Lane1: A549 cell
lysate; Lane2: Porcine Heart lysate
Primary Ab: 0.3?g/ml Rabbit Anti
Human AIF Antibody
Second Ab: 0.2µg/mL HRP-Linked
Caprine Anti-Rabbit IgG Polyclonal
Antibody

Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: AIF knockout A549 cell lysate;
Predicted MW: 26~35,66kd
Observed MW: 66kd
Primary Ab: 3µg/ml Rabbit Anti-Human
AIF Antibody
Second Ab: 0.2µg/mL HRP-Linked
Caprine Anti-Rabbit IgG Polyclonal
Antibody

Western Blot; Sample: Lane1: Hela cell
lysate; Lane2: Bovine Heart lysate
Primary Ab: 0.3?g/ml Rabbit Anti-
Human AIF Antibody
Second Ab: 0.2µg/mL HRP-Linked
Caprine Anti-Rabbit IgG Polyclonal
Antibody

[ IMPORTANT NOTE ] 
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.
本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。