DPM1,DPM1蛋白抗体

产品编号HZ-14419R
英文名称DPM1
中文名称DPM1蛋白抗体
别 名CDGIE; dolichol monophosphate mannose synthase; Dolichol phosphate mannose synthase; Dolichol-phosphate mannose synthase; Dolichol-phosphate mannosyltransferase; Dolichyl phosphate beta D mannosyltransferase; dolichyl phosphate mannosyltransferase polypeptide 1; dolichyl phosphate mannosyltransferase polypeptide 1 catalytic subunit; Dolichyl-phosphate beta-D-mannosyltransferase; DPM synthase; DPM1; DPM1_HUMAN; Mannose P dolichol synthase; Mannose-P-dolichol synthase; MPD synthase; MPDS.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
DPM1,DPM1蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量30kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human DPM1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
DPM1,DPM1蛋白抗体PubMedPubMed
产品介绍background:
Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. [provided by RefSeq, Jul 2008]

Function:
Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.

Subcellular Location:
Endoplasmic reticulum.

DISEASE:
Defects in DPM1 are the cause of congenital disorder of glycosylation type 1E (CDG1E) [MIM:608799]. CDGs are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under-glycosylated serum glycoproteins. CDG1E is an autosomal recessive disorder, characterized by severe developmental delay, hypotnia, seizures, and dysmorphic features.

Similarity:
Belongs to the glycosyltransferase 2 family.

Gene ID:
8813

DPM1,DPM1蛋白抗体Database links:
Entrez Gene: 8813 Human
Entrez Gene: 534097 Cow
Entrez Gene: 13480 Mouse
Entrez Gene: 100124379 Pig
Entrez Gene: 296394 Rat
Omim: 603503 Human
SwissProt: Q1JQ93 Cow
SwissProt: O60762 Human
SwissProt: O70152 Mouse
SwissProt: A5GFZ5 Pig
Unigene: 654951 Human
Unigene: 422657 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-DPM1 Polyclonal Antibody, Unconjugated(HZ-14419R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining