VAMP8,囊泡相关膜蛋白8抗体

产品编号HZ-11395R
英文名称VAMP8
中文名称囊泡相关膜蛋白8抗体
别 名EDB; Endobrevin; VAMP 5; VAMP-5; VAMP 8; VAMP-8; VAMP5; VAMP8; VAMP8_HUMAN; Vesicle associated membrane protein 8; Vesicle-associated membrane protein 8.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 细胞分化
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
VAMP8,囊泡相关膜蛋白8抗体产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量11kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human VAMP8 (32-80aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
VAMP8,囊泡相关膜蛋白8抗体PubMedPubMed
产品介绍background:
Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAPs (soluble NSF attachment proteins) and Synaptotagmin. Endobrevin, also designated VAMP-8 or ED, is a 100 amino acid single-pass type IV membrane protein that belongs to the synaptobrevin family. Similar in sequence to the synaptobrevins, endobrevin is abundantly expressed in kidney, moderately expressed in heart and spleen, and slightly expressed in brain, thymus and liver. Endobrevin interacts specifically with the SNAPs, most likely through an endobrevin-containing SNARE complex.

Function:
Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.

Subunit:
Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (By similarity). Interacts with STX8 (By similarity).

Subcellular Location:
Membrane; Single-pass type IV membrane protein.

VAMP8,囊泡相关膜蛋白8抗体Tissue Specificity:
Platelets.

Similarity:
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.

Database links:
Entrez Gene: 8673 Human
Entrez Gene: 22320 Mouse
Entrez Gene: 83730 Rat
Omim: 603177 Human
SwissProt: Q9BV40 Human
SwissProt: O70404 Mouse
SwissProt: Q9WUF4 Rat
Unigene: 714302 Human
Unigene: 1838 Mouse
Unigene: 82672 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-VAMP8 Polyclonal Antibody, Unconjugated(HZ-11395R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining