UBE2A,泛素结合酶E2蛋白A抗体

产品编号HZ-8375R
英文名称UBE2A
中文名称泛素结合酶E2蛋白A抗体
别 名BHR6A; hHR6A; HR6A; mHR6A; RAD6 homolog A; RAD6A; RAD6B; UBC-1; UBC2; UBC6; UBCD6; UBE2A; UBE2A_HUMAN; UBE2B; Ubiquitin carrier protein A; Ubiquitin carrier protein; Ubiquitin conjugating enzyme E2 17 kDa; Ubiquitin conjugating enzyme E2 21.5 kDa; Ubiquitin conjugating enzyme E2 A; Ubiquitin protein ligase A; Ubiquitin-conjugating enzyme E2 A; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-conjugating enzyme E2-21.5 kDa; Ubiquitin-protein ligase A.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Cow,
UBE2A,泛素结合酶E2蛋白A抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量17kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human UBE2A/UBE2B/RAD6
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
UBE2A,泛素结合酶E2蛋白A抗体PubMedPubMed
产品介绍background:
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (Ubiquitin-conjugating enzyme E2 A) and UBE2B (Ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to postreplication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.

Function:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.

Subunit:
Interacts with RAD18 and WAC.

DISEASE:
Defects in UBE2A are the cause of mental retardation syndromic X-linked Nascimento-type (MRXSN) [MIM:300860]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSN features include dysmorphic facies, hirsutism, skin and nails abnormalities, obesity, speech anomalies and seizures.

UBE2A,泛素结合酶E2蛋白A抗体Similarity:
Belongs to the ubiquitin-conjugating enzyme family.

Gene ID:
7319

Database links:
Entrez Gene: 395672 Chicken
Entrez Gene: 282107 Cow
Entrez Gene: 7319 Human
Entrez Gene: 22209 Mouse
Entrez Gene: 298317 Rat
Entrez Gene: 398788 Xenopus laevis
Entrez Gene: 797853 Zebrafish
Omim: 312180 Human
SwissProt: P49459 Human
SwissProt: P63146 Human
SwissProt: Q9Z255 Mouse
Unigene: 379466 Human
Unigene: 395649 Mouse

UBE2A,泛素结合酶E2蛋白A抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.