Product: Anti-JAM-1, clone M.Ab.F11
Cat. No.: MC-199 (100 μg)
Synonyms:
Human platelet F11-Receptor (F11R)
Description:
Junctional adhesion molecule-1 (JAM-1) is a cell
adhesion molecule (CAM). JAM-1 is a member
of the immunoglobulin superfamily found on the
surface of human platelets and at intercellular
junctions of endothelial cells and epithelial cells.
JAM-1 belongs together with JAM-2 and JAM-3
to a family of adhesion proteins with a V-C2
immunoglobulin domain organization. JAM plays
an important role in tight junctions where it is
involved in cell-to-cell adhesion through
homophilic interaction. It co-distributes with
other tight junction components such as ZO-1,
7H6 antigen, cingulin and occludin. In humans
JAM-1 plays a role in platelet aggregation,
secretion, adhesion and spreading.
JAM-1 is a membrane protein involved in 2
distinct processes initiated on the platelet
surface. Antibody-induced platelet aggregation
and secretion both depend on FcgammaRII and
GPIIb/IIIa intergrin, a process that may be
involved in pathophysiological processes
associated with certain thrombocytopenias.
Antibody mediated platelet adhesion
independent from FcgammaRII or fibrinogen
receptor and that appears to place a role in
physiological processes associated with platelet
adhesion and aggregation. A physiological role
for the JAM-1 protein was demonstrated by its
phosphorylation after the stimulation of platelets
by thrombin and collagen. A pathophysiological
role for the JAM-1 antibodies in patients with
thrombocytopenia. Adhesion of platelets through
the F11R resulted in events characteristic of the
action of cell adhesion molecules (CAMs).
Recent data suggests a role for JAM-1 in the
adhesion of platelets to cytokine-inflamed
endothelial cells and thus in thrombosis and
atherosclerosis induces in non-denuded blood
vessels by inflammatory processes.
Specificity:
Recognizes human JAM-1.
Ig Isotype:
Mouse IgG1
Species Reactivity:
Human. Others not tested.
Format:
1 mL of 100 μg/mL 0.2 μm filtered antibody
solution in PBS containing 0.1 protein
stabilizer.
Storage:
Store at 4°C, short term. Store at –20°C long
term. Aliquot to avoid freeze/thaw cycles.
Applications and Suggested Dilutions:

Flow cytometry: Use at a 1:10 dilution.

Immunoprecipitation

Functional application: Antibody directly
stimulates the aggregation and granule
secretion of human platelets.

Western blotting: Use at a 1:10 dilution
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.