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Vaccinia Virus (Lister strain) Quantitated Viral DNA PCR control.

For research use only. Not for use in diagnostic procedures.

ABI’s Quantitated PCR Controls can be used to generate standard curves for quantitative PCR assays and as positive controls in nucleic acid amplification reactions. Quantitated PCR Controls for viruses, bacteria, and other organisms such as Toxoplasma gondii and Mycoplasma pneumoniae are available.

ABI’s Quantitated PCR Controls are purified from one of the following sources:

• Intact Viral Particles, Parasites or Mollicutes
• Cultured Bacteria: pelleted bacteria from liquid, plate, or slant culture
• Cloned viral DNA

Quantitated PCR Controls are supplied in volumes of 250 µL at 1-2 x 10⁴ DNA copies/µL (except for BK and JC polyomaviruses, and Porcine Circovirus Type 2 (PCV2), which are supplied at 1-2 x 10⁵ DNA copies/µL). Quantitation with different instruments and by different methods may not give the same copy number results.

Note: ABI’s Quantitated PCR Controls are intended for use as positive PCR quantitative standards for the organism in question. Due to the nature of these products, ABI cannot guarantee their suitability as extraction controls. Additionally, due to the extreme sensitivity of detection in PCR reactions, and since no method of purification can guarantee the complete absence of extraneous agents, PCR controls are not intended for use as negative controls for other organisms.

ABI’s PCR Controls are for research use only and are not for use in diagnostic assays.

Details（注意事项）:

Shipping and Storage: This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.

Recommendations: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.CAUTION: ABI does not recommend storage of dilutions of quantitative DNA Controls under any conditions. All dilutions should be made immediately before use and used promptly. We have observed that dilutions used for standard curves may tend to “lose” copy number with time (sometimes a matter of an hour or so after dilution), especially at dilutions less than 100-1000 copies per microliter.

Safe Handling Recommendation: The DNA extraction procedure used has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested for infectivity and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.

FAO（常见问题）：

 How are Quantitated DNA controls supplied? Quantitated DNA controls are supplied at 1-2 × 10⁴ copies/µL (with the exception of BK and  JC polyomaviruses and PCV2, which are supplied at 1-2 × 10⁵ copies/µL).  The copy number of  Quantitated DNA controls is determined by real-time PCR.

How can dilutions of Quantitated DNA controls be stored? ABI does not recommend storing dilutions of these controls under any conditions.  All  dilutions should be made immediately before use and used promptly.  We have observed that  dilutions used for standard curves typically “lose” copy number with time — sometimes as soon  as an hour or so after dilution.  This is especially common at dilutions less than 100-1000 copies  per microliter.

Applications for use（适用范围）:

Quality control testing includes（质量控制检测包括）:

Certificate of Analysis（分析报告）: