Introduction
Lipid peroxidation is a well-defined mechanism of cellular damage in animals and plants. Lipid
peroxides are unstable indicators of oxidative stress in cells that decompose to form more complex and
reactive compounds such as Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), natural biproducts
of lipid peroxidation. Oxidative modification of lipids can be induced in vitro by a wide array
of pro-oxidant agents and occurs in vivo during aging and in certain disease conditions. Measuring the
end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage.
These aldehydic secondary products of lipid peroxidation are generally accepted markers of oxidative
stress. Both MDA and HNE have been shown to be capable of binding to proteins and forming stable
adducts, also termed advanced lipid peroxidation end products. These modifications of proteins by
MDA or HNE can cause both structural and functional changes of oxidized proteins.
Cell Biolabs’ MDA Immunoblot Kit is a simple and complete system for the detection of MDAprotein
adducts. The kit includes antibodies for the detection of MDA in samples and an MDA-BSA
Immunoblot Control for use as a positive control. Each kit provides sufficient quantities to perform at
least 10 blots (7.5 cm x 8.5 cm).