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Dipeptidyl-peptidase II ( DPPII / DPP7 ) Antibody Datasheet
 
 
 
DPPII / DPP7抗体产品信息
 
 
 
 
免疫原 : 
 
 
Human cell-derived DPPII protein ( Catalog #10748-H08H )
 
 
 
 
 
Clone ID :
 
 
 
5H11H7B8
 
 
 
 
抗体宿主 :
 
Mouse IgG1
 
 
 
缓冲液 :
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
制备方法 : 
 
 
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human DPPII / DPP7 ( rhDPPII; Catalog #10748-H08H; NP_037511.2; Met 1-Leu 492 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 
 
 
 
 
DPPII / DPP7抗体使用指南
 
 
 
 
 
特异性 : 
 
 
 
Human DPPII
 
 
 
 
 
 
No cross-reactivity with human cell lysate (293 cell line) in WB and ELISA.
 
 
 
Western blot :
 
This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect DPPII / DPP7 in WB. Using a DAB detection system, the detection limit for DPPII / DPP7 is approximately 4ng/lane and 100ng/lane under non-reducing conditions and reducing conditions.
 
 
 
储存方法 :
 
This antibody can be stored at 2℃-8℃ for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
 Avoid repeated freeze-thaw cycles.
 
 
 
Image : 
 
 
 
The detection limit for DPPII is approximately 100ng /lane under reducing conditions
 
 
 
 
 
 
 
 
The detection limit for DPPII is approximately 4ng /lane under non-reducing conditions
 
 
 
 
 
DPPII / DPP7 Antibody Related Products & Topics
 
 
相关研究领域:
 
Enzyme>>Protease & Regulator>>Serine Protease & Regulator>>DPPIV Family>>DPP7/DPPII/DPP2
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
DPP7/DPPII/DPP2
 
Human
 
DPP7/DPPII/DPP2 Protein, Recombinant 
 
10748-H08H
 
 
 
 
抗体:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
Human DPP7/DPPII/DPP2
 
WB
 
Mouse Monoclonal Antibody
 
10748-MM02
 
 
 
Human DPP7/DPPII/DPP2
 
ELISA
 
Mouse Monoclonal Antibody
 
10748-MM03
 
 
 
Human DPP7/DPPII/DPP2
 
ELISA
 
Rabbit Polyclonal Antibody
 
10748-RP01
 
 
 
Human DPP7/DPPII/DPP2
 
WB, ELISA
 
Rabbit Polyclonal Antibody (Antigen Affinity Purified)
 
10748-RP04
 
 
 
 
 
 DPPII / DPP7抗体背景综述
 
 
Dipeptidyl-peptidase II (DPPII), also known as quiescent cell proline dipeptidase (QPP) and dipeptidyl-peptidase 7 (DPP7), is a post-proline cleaving amino peptidase expressed in quiescent lymphocytes. As an intracellular protease localized to the vesicular system, it releases preferably N-terminal dipeptides from oligopeptides with Pro or Ala in the penultimate position at acidic pH. The proline-specific DPPs like DPP7, fibroblast activation protein alpha (FAP), DPPIV, DPP8 and DPP9, on the basis of their preference for cleavage after X-Pro, are likely to be involved in Pro-specific N-terminal processing regulating an increasing number of biological processes. These DPPs are emerging as an important protease family involved in the regulation of signaling by peptide hormones. The purified rhDPP7 is active against Lys-Pro-AMC and Lys-Ala-AMC. Its activity against Lys-Pro-AMC is approximately 10-fold of that against Lys-Ala-AMC under identical conditions.
 
 
参考文献
 
 
1.  Underwood, R. et al., 1999, J. Biol. Chem. 274: 34053-8.
 
2.  Zhan, H. et al., 2002,  Histochem. J. 33 (9-10): 511-21.
 
3.  Rosenblum, JS. et al., 2003, Curr. Opin. Chem. Biol. 7: 496-504.
 
4.  Maes, MB. et al., 2005, Biochem. J. 386: 315-24.
 
5.  Lankas, GR. et al., 2005, Diabetes 54: 2988-94.
 
6.  Maes, MB. et al., 2007, Clin Chim Acta. 380 (1-2): 31-49.
 
 
 
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