ALT1,谷丙氨酸氨基转移酶1抗体

产品编号HZ-2532R
英文名称ALT1
中文名称谷丙氨酸氨基转移酶1抗体
别 名Alanine aminotransferase; AAT 1; AAT1; Alanine aminotransferase 1; alanine aminotransferase; ALT 1; ALT1; Glutamic alanine transaminase 1; Glutamic pyruvate transaminase; Glutamic pyruvic transaminase 1; Glutamic-alanine transaminase; GPT 1; GPT; GPT1; SGPT; ALAT1_HUMAN.
规格价格0.1ml 0.2ml 
说 明 书0.1ml 0.2ml
研究领域激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Pig, Cow,
ALT1,谷丙氨酸氨基转移酶1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量55kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ALT1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ALT1,谷丙氨酸氨基转移酶1抗体PubMedPubMed
产品介绍background:
Alanine Transaminase [glutamic pyruvate transaminase - GPT] participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Pyridoxal phosphate is a known cofactor. Expression is induced by glucocorticoids. This enzyme is present in a high concentration in liver tissue and it's release into the blood indicates liver disease or damage.

Function:
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.

Similarity:
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Gene ID:
2875

ALT1,谷丙氨酸氨基转移酶1抗体Database links:
Entrez Gene: 539188 Cow
Entrez Gene: 2875 Human
Entrez Gene: 76282 Mouse
Entrez Gene: 81670 Rat
Omim: 138200 Human
SwissProt: A4IFH5 Cow
SwissProt: P24298 Human
SwissProt: Q8QZR5 Mouse
SwissProt: P25409 Rat
Unigene: 103502 Human
Unigene: 30130 Mouse
Unigene: 6318 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ALT1 Polyclonal Antibody, Unconjugated(bs-2532R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ALT1 Polyclonal Antibody, Unconjugated(bs-2532R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining