PCR Array

克罗恩病PCR芯片可用于研究在肠道炎症（IBD）中差异表达的84个基因的表达。克罗恩病与溃疡性结肠炎（UC）都是常见的肠道炎症。肠炎的主要症状包括腹痛、呕吐、腹泻等，持续性的炎症会导致体重减轻，引发结肠或直肠癌，甚至因此切除器官。在炎症发生和恶化过程中，作为细胞外重构酶的炎症细胞因子，调节免疫反应的T细胞均发挥了重要作用，是研究各种肠道炎症的生物标志物。早期的表达谱芯片研究发现了与克罗恩氏病和UC先相关的基因，这些基因均已收录在这一芯片上。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测肠道炎症相关基因的表达。
Apoptosis: CASP1 (ICE), CCL2 (MCP-1), EGR3, IL1B, IL2RA (CD25), IL6, ITGB2, LCN2 (NGAL), SOD2, TNF. Inflammation: C3, CCL11 (Eotaxin), CCL2 (MCP-1), CCL20 (MIP-3A), CCL25 (TECK), CCL5 (RANTES), CCR1, CCR2, CCR5, CX3CL1, CXCL1, CXCL10 (INP10), CXCL11 (I-TAC/IP9), CXCL2, CXCL3, CXCL9 (MIG), CXCR1 (IL8RA), FPR1, IL13, IL17A, IL1B, IL2RA (CD25), IL23A, IL5, IL6, IL8, ITGB2, LTB, LYZ, S100A8, S100A9, SAA1, SELE, STAT3, TNF. Immunity: Humoral: CCL2 (MCP-1), IL6, DEFA5, DEFA6, LYZ. Innate: C3, C4BPB, CASP1, CD55, CR2, IL1RN, IRF5, ISG15 (G1P2), LCN2 (NGAL), NOD2, NOS2 (iNOS), SAA1, TYK2. Adaptive: CCL11 (Eotaxin), CCR2, CCR5, CCR9, CXCR3, HLA-DQA1, HLA-DRA, HLA-DRB1, IFNG, IL13, IL17A, IL2RA (CD25), IL5, IL6, IRF5, STAT1. Adhesion & Remodeling: CCL2 (MCP-1), CCR1, CCL11 (Eotaxin), CSTA, CX3CR1, CXCL12 (SDF1), CXCR3, ITGB2, MMP1, MMP10, MMP3, MMP7, PECAM1, SELE, SELL (LECAM1), TIMP1, VWF. Unfolded Protein Response: HSP90B1 (TRA1), HSPA5 (GRP78), UBD. Digestive Secreted Proteins: GCG, MUC1, REG1A, REG1B, TFF1 (pS2). Metabolism: ALDOB, PCK1, TDO2. Other Genes: ABCB1 (MDR1), ATG16L1, CHI3L1, EDN3, NR3C2