TRIM41,环指蛋白调节蛋白激酶C蛋白抗体

产品编号HZ-9151R
英文名称TRIM41
中文名称环指蛋白调节蛋白激酶C蛋白抗体
别 名RINCK; RING finger protein that interacts with C kinase; Trim41; Tripartite motif containing 41; TRI41_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Sheep,
TRIM41,环指蛋白调节蛋白激酶C蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量72kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human TRIM41
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
TRIM41,环指蛋白调节蛋白激酶C蛋白抗体PubMedPubMed
产品介绍background:
Tripartite motif-containing protein 41 (TRIM41), also known as RINCK, is a 630 amino acid member of the TRIM family, also known as the RING-B-box coiled-coil (RBCC) family. Members of the RBCC family have an N-terminal RING finger, followed by one or two zinc-binding domains (B-box domains), a leucine coiled-coil region and a variable C-terminal domain. Localized to both the nucleus and cytoplasm, TRIM41 associates with protein kinase C (PKC) through the C1A domain of PKC. Studies have shown that overexpression of TRIM41 reduces the levels of PKC in cells, whereas knockdown of TRIM41 leads to increased levels of PKC. Thus, it is hypothesized that TRIM41 plays a role in regulating PCK levels in cells, specifically through the ubiquitination of PKC. Four isoforms of TRIM41 exist as a result of alternative splicing events.

Function:
Functions as an E3 ligase that catalyzes the ubiquitin-mediated degradation of protein kinase C.

Subunit:
Interacts with PRKCA.

Subcellular Location:
Cytoplasm. Nucleus.

TRIM41,环指蛋白调节蛋白激酶C蛋白抗体Tissue Specificity:
Expressed in multiple tissues with the highest levels in heart and skeletal muscle.

Post-translational modifications:
Auto-ubiquitinated.

Similarity:
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.

Gene ID:
90933

Database links:
Entrez Gene: 90933 Human
Entrez Gene: 211007 Mouse
Entrez Gene: 303088 Rat
Omim: 610530 Human
SwissProt: Q8WV44 Human
SwissProt: Q5NCC3 Mouse
Unigene: 441488 Human
Unigene: 794 Mouse

TRIM41,环指蛋白调节蛋白激酶C蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-TRIM41 Polyclonal Antibody, Unconjugated(HZ-9151R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining