TRPM7,瞬时受体电位离子通道蛋白7抗体

产品编号HZ-9044R
英文名称TRPM7
中文名称瞬时受体电位离子通道蛋白7抗体（M亚家族）
别 名CHAK 1; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; FLJ20117; FLJ25718; homolog of mouse transient receptor potential-phospholipase C-interacting kinase; Long transient receptor potential channel 7; LTRPC 7; LTRPC ion channel family member 7; LTrpC-7; LTRPC7; transient receptor potential cation channel subfamily M member 7; TRP PLIK; TRP-PLIK; TRPM 7; TRPM7; TRPM7_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 神经生物学 信号转导 通道蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
TRPM7,瞬时受体电位离子通道蛋白7抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量180, 205kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human TRPM7
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
TRPM7,瞬时受体电位离子通道蛋白7抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam.[provided by RefSeq, May 2010].

Function:
Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).

Subunit:
Homodimer. Interacts with PLCB1. Forms heterodimers with TRPM6.

Subcellular Location:
Membrane; Multi-pass membrane protein

Post-translational modifications:
Autophosphorylated (By similarity).

TRPM7,瞬时受体电位离子通道蛋白7抗体DISEASE:
Defects in TRPM7 are a cause of susceptibility to amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 (ALS-PDC1) [MIM:105500]; also called amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam or Guam disease. Amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 is a neurodegenerative disorder characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. Both diseases are known to occur in the same kindred, the same sibship and even the same individual.

Similarity:
In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
Contains 1 alpha-type protein kinase domain.

Database links:
Entrez Gene: 54822 Human
Entrez Gene: 58800 Mouse
Entrez Gene: 679906 Rat
Omim: 605692 Human
SwissProt: Q96QT4 Human
SwissProt: Q923J1 Mouse
SwissProt: Q925B3 Rat
Unigene: 512894 Human
Unigene: 244705 Mouse
Unigene: 86991 Rat

TRPM7,瞬时受体电位离子通道蛋白7抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat choroid tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-TRPM7 Polyclonal Antibody, Unconjugated(HZ-9044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat melanoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-TRPM7 Polyclonal Antibody, Unconjugated(HZ-9044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-TRPM7 Polyclonal Antibody, Unconjugated(HZ-9044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining