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上海信裕生物科技有限公司
APOB抗体

APOB抗体

商家询价

产品名称： APOB抗体

英文名称： APOBAntibody

产品编号： XY20578-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
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APOB抗体

Applications
In-house applications ELISA, IHC, WB, IF     Application not tested? Please see our Guarantee.
Species specificity Human, Mouse; other species not tested.
Images
Prev1NextView more validation data
  • 20578-1-AP;human liver tissueImmunohistochemistry of paraffin-embedded human liver slide using 20578-1-AP( APOB Antibody) at dilution of 1:50
  • 20578-1-AP;mouse liver tissuemouse liver tissue were subjected to SDS PAGE followed by western blot with 20578-1-AP( APOB Antibody) at dilution of 1:600
  • 20578-1-AP;HepG2 cellsImmunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 20578-1-AP( APOB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in Mouse liver tissue, HEK-293 cells, HepG2 cells, L02 cells
Positive IHC detected in Human liver tissue
Positive IF detected in HepG2 cells
Recommended dilution WB : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200
APOB抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen Peptide Full name Apolipoprotein B (including Ag(x) antigen)
Calculated molecular
weight		 516 kDa Observed molecular 
weight		 130-150 kDa, 200-250 kDa
GenBank accession 
number		 NM_000384 Gene ID (NCBI) 338
Gene symbol APOB Synonyms Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B, Apolipoprotein B 100, FLDB
BackgroundAPOB抗体

The apolipoprotein B (ApoB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The ApoB gene encodes two different isoproteins through mRNA editing, ApoB-48 and ApoB-100. ApoB-100 is synthesized exclusively by the liver and is essential for the assembly of VLDL in the liver. ApoB-48 is synthesized exclusively by the small intestine and is essential for the assembly of chylomicrons in the intestine. ApoB-48 and ApoB-100 share a common N-terminal sequence, but ApoB-48 lacks ApoB-100's C-terminal LDL receptor binding region. It is well established that ApoB-100 levels are associated with coronary heart disease. This antibody recognizes both of ApoB-48 and ApoB-100.APOB抗体

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