FITC标记的ECSIT蛋白抗体
产品名称： FITC标记的ECSIT蛋白抗体
英文名称： Anti-ECSIT/FITC
产品编号： HZ-14488R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
上海沪震实业有限公司
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
- 传真 : 点击查看
- 邮箱 : www.shzbio.net
- 二维码 : 点击查看
Rabbit Anti-ECSIT/FITC Conjugated antibody
FITC标记的ECSIT蛋白抗体
| 英文名称 | Anti-ECSIT/FITC |
| 中文名称 | FITC标记的ECSIT蛋白抗体 |
| 别 名 | Ecsit; ECSIT homolog; ECSIT protein; ECSIT_HUMAN; Evolutionarily conserved signaling intermediate in Toll pathway; Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial; Evolutionarily conserved signaling intermediate in Toll pathways; Likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved; Protein SITPEC; Signaling intermediate in Toll pathway evolutionarily conserved; SITPEC; SITPEC protein. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Cow, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ECSIT |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: ECSIT is a 431 amino acid ubiquitously expressed protein that plays an important role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. Within the Toll pathway, ECSIT regulates MEKK1 processing for activation of NFκB, a major event leading to initiation of the innate immune response. In the mitochondria, ECSIT interacts with NDUFAF1 and assists in the formation of NADH:ubiquinone oxidoreductase (complex I), an extremely complicated multiprotein complex located in the inner mitochondrial membrane that functions in the transport of electrons from NADH to ubiquinone. Knockdown of ECSIT results in severely impaired complex I assembly and disturbed mitochondrial function. There are two isoforms of ECSIT that are produced as a result of alternative splicing events Function: Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity). Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase. Subunit: Interacts with MAP3K1, SMAD4 and TRAF6. Interacts with SMAD1 only after BMP4-treatment (By similarity). Part of the mitochondrial complex I assembly (MCIA) complex. The complex comprises at least TMEM126B, NDUFAF1, ECSIT, and ACAD9 (By similarity). Interacts with NDUFAF1. Subcellular Location: Cytoplasm. Nucleus. Mitochondrion. Similarity: Belongs to the ECSIT family. Database links: Entrez Gene: 51295 Human Entrez Gene: 26940 Mouse Entrez Gene: 300447 Rat Omim: 608388 Human SwissProt: Q9BQ95 Human SwissProt: Q9QZH6 Mouse SwissProt: Q5XIC2 Rat Unigene: 515146 Human Unigene: 425568 Mouse Unigene: 8455 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
ECSIT是一种431氨基酸普遍表达的蛋白，在Toll受体激活的细胞质信号转导级联反应中起着重要作用。在Toll通路中，ECSIT调节MEKK1处理以激活NFκB，这是导致先天免疫应答启动的主要事件。在线粒体中，ECSIT与NDUFAF1相互作用并协助NADH：泛醌氧化还原酶（复合物I）的形成，NADH：泛醌氧化还原酶（复合物I）是位于线粒体内膜的一种极其复杂的多蛋白复合物，在电子从NADH到泛醌的转运中起作用。EcSIT的敲除导致严重受损的复杂I组装和线粒体功能紊乱。作为替代剪接事件的结果，产生了两种EcSIT的异构体。