phospho-APBB1,磷酸化铁蛋白Fe65抗体

产品编号HZ-5165R
英文名称phospho-APBB1 (Ser175)
中文名称磷酸化铁蛋白Fe65抗体
别 名Adaptor protein FE65a2; Amyloid beta (A4) precursor protein binding family B member 1; Amyloid beta A4 precursor protein binding family B; Amyloid beta A4 precursor protein binding family B member 1; Amyloid beta precursor protein binding family B member 1; APBB 1; APBB1; FE 65; Fe65 protein; MGC 9072; MGC9072; Protein Fe65; RIR; Stat like protein; Fe-65 Protein; APBB1_HUMAN.
规格价格0.1ml
说 明 书0.1ml
产品类型磷酸化抗体
phospho-APBB1,磷酸化铁蛋白Fe65抗体研究领域肿瘤 细胞生物 免疫学 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量77kDa
细胞定位细胞核 细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human APBB1 around the phosphorylation site of Ser175 [LS(p-S)PP]
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-APBB1,磷酸化铁蛋白Fe65抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Function:
Transcription coregulator that can have both coactivatorand corepressor functions. Adapter protein that forms atranscriptionally active complex with the gamma-secretase-derivedamyloid precursor protein (APP) intracellular domain. Plays acentral role in the response to DNA damage by translocating to thenucleus and inducing apoptosis. May act by specifically recognizingand binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph)at double-strand breaks (DSBs), recruiting other pro-apoptosisfactors such as MAPK8/JNK1. Required for histone H4 acetylation atdouble-strand breaks (DSBs). Its ability to specifically bindmodified histones and chromatin modifying enzymes such asKAT5/TIP60, probably explains its trancription activation activity.Function in association with TSHZ3, SET and HDAC factors as atranscriptional repressor, that inhibits the expression of CASP4.Associates with chromatin in a region surrounding the CASP4transcriptional start site(s).

phospho-APBB1,磷酸化铁蛋白Fe65抗体Subunit:
Component of a complex, at least composed of APBB1,RASD1/DEXRAS1 and APP. Interacts (via PID domain 2) with APP (withthe intracellular domain of the beta-amyloid precursor protein).Interacts (via PID domain 2) with RASD1/DEXRAS1; impairs thetrancription activation activity. Interacts (via PID domain 1) withKAT5/TIP60. Interacts (via the WW domain) with the proline-richregion of APBB1IP. Interacts with TSHZ1 and TSHZ2 (By similarity).Interacts (via the WW domain) with histone H2AX (whenphosphorylated on 'Tyr-142') and the proline-rich region of ENAH.Interacts with MAPK8. Interacts (via PID domain 1) with TSHZ3 (viahomeobox domain). Interacts with SET. Found in a trimeric complexwith HDAC1 and TSHZ3; the interaction between HDAC1 and APBB1 ismediated by TSHZ3. Interacts (via WWW domain) with NEK6. Interacts(via WWW domain) with ABL1.

Subcellular Location:
Cell membrane. Cytoplasm. Nucleus. Cellprojection, growth cone (By similarity). Nucleus speckle.Note=Colocalizes with TSHZ3 in axonal growth cone (By similarity).In normal conditions, it mainly localizes to the cytoplasm, while asmall fraction is tethered to the cell membrane via its interactionwith APP. Following exposure to DNA damaging agents, it is releasedfrom cell membrane and translocates to the nucleus. Nucleartranslocation is under the regulation of APP. Colocalizes withTSHZ3 in the nucleus. Co-localizes with NEK6 at the nuclearspeckles. Phosphorylation at Ser-610 by SGK1 promotes itslocalization to the nucleus

Tissue Specificity:
Highly expressed in brain; strongly reduced inpost-mortem elderly subjects with Alzheimer disease.

Post-translational modifications:
Phosphorylation at Ser-610 by SGK1 promotes its localizationto the nucleus (By similarity). Phosphorylated following nucleartranslocation. Phosphorylation at Tyr-547 by ABL1 enhancestranscriptional activation activity and reduces the affinity forRASD1/DEXRAS1.

Similarity:
Contains 2 PID domains.
Contains 1 WW domain.

Database links:
UniProtKB/Swiss-Prot: O00213.2 (human)

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Fe65 蛋白(又称作Amyloid beta A4 precursor protein-binding family B member 1)可能与细胞内的APP结构域结合调节内源性的APP。