Recombinant Human LIF

Catalog Number:

300-05

Synonyms:

Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI)

Description:

LIF是一种多效性细胞因子，由多种类型的细胞，包括T细胞，骨髓单核细胞系，成纤维细胞，肝细胞，心脏和黑色素瘤等产生。LIF通过抑制自发分化来促进胚胎干细胞(ES细胞)的长期维持。LIF的其它活性包括刺激肝脏急性期蛋白的合成，促进胆碱能神经的分化，以及通过抑制脂肪细胞中脂蛋白脂酶的活性而抑制脂肪生成。人与小鼠的LIF间具有种属交叉反应活性。重组人LIF是一个分子量为19.6 kDa的蛋白质，含有180个氨基酸残基。

AA Sequence:

SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF

Source:

E.coli

Purity:

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity:

Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED₅₀ is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.

Research Interests:

	Diabetes / Weight Regulation		Immune System
	Lipid Metabolism		Proliferation
	Stem Cells & Differentiation

Country of Origin:

USA

Research Use Only. Not for use in diagnostic or therapeutic procedures.