SV2B,突触泡蛋白2B抗体

产品编号HZ-11365R
英文名称SV2B
中文名称突触泡蛋白2B抗体
别 名Synaptic vesicle protein 2B; SV2B_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Rabbit,
SV2B,突触泡蛋白2B抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量77kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human SV2B
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
SV2B,突触泡蛋白2B抗体PubMedPubMed
产品介绍background:
In all vertebrates, SV2 proteins are abundant, hydrophobic, membrane glycoproteins that are expressed as two major isoforms, SV2A and SV2B, and one minor isoform, SV2C. SV2 proteins are differentially expressed in the brain and are present on all synaptic vesicles, independent of transmitter type. SV2A is abundantly expressed in the subcortex, specifically in the synaptic vesicles of all presynaptic nerve terminals, and also in most neuroendocrine secretory granules. SV2B displays a more restricted pattern of expression in that it is only present on a small subset of synapses in the hippocampus and cortex. SV2A and SV2B are funtionally redundant and are required for maintaining normal brain function in vertebrates. SV2A and SV2B mediate synaptic transmission by regulating cytoplasmic Ca2+ levels in the nerve terminal during repetitive stimulation.

Function:
SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. While SV2A is expressed ubiquitously throughout the brain, SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern.

Subunit:
Interacts with SYT1 in a calcium-independent manner. Forms a complex with SYT1, syntaxin-1 and SNAP25 (By similarity).

Subcellular Location:
Cytoplasmic vesicle, secretory vesicle. Synaptic vesicle membrane; Multi-pass membrane protein.

SV2B,突触泡蛋白2B抗体Post-translational modifications:
N-glycosylated (By similarity).
The N-terminal cytoplasmic domain is phosphorylated by CK1 (By similarity).

Similarity:
Belongs to the major facilitator superfamily.

Database links:
UniProtKB/Swiss-Prot: Q7L1I2.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.