辅助T细胞分化PCR芯片可用于研究前体T细胞分化成特定功能亚群的84个关键调控基因的表达。研究最为广泛的两个辅助T细胞亚群Th1和Th2，分别介导细胞和体液免疫反应。大量研究结果表明两者其一在各种免疫紊乱性疾病如过敏、哮喘、自身免疫、糖尿病和类风湿性关节炎中起着主导作用。该芯片可检测的基因包括调控Th1和Th2细胞分化的细胞因子、细胞因子受体、转录因子和其它信号分子以及各亚型的标志物。通过对T细胞RNA样本的芯片研究可以很好地将实验诱导的细胞免疫和体液免疫反应与T细胞亚群类型相关联，得到的结果同样有助于对研究者模型中T细胞分化分子机制和生物学通路的深入研究。使用实时定量PCR，可以简单可靠地同时分析辅助T细胞分化相关基因的表达。
Cytokines & Receptors: CCL5 (RANTES), CCL7 (MCP-3), CCR3, CCR4, CCR5, CCR6, IL12B, IL12RB2, IL13, IL13RA1, IL17A, IL17RE, IL18, IL18R1, IL18RAP, IL1R1, IL1R2, IL1RL1, IL2, IL21, IL2RA, IL4, IL4R, IL5, IL9, TNF. T Helper 1 Subtype Markers: CCR5, HAVCR2, IGSF6, IL12B, IL18, IRF1, SOCS1, SOCS5, TLR4, TLR6, TNF. T Helper 2 Subtype Markers: CCL5 (RANTES), CCL7 (MCP-3), CCR3, CCR4, CEBPB, GFI1, GPR44, ICOS, IL13RA1, IL4R, JAK1, NFATC1, NFATC2. Transcription Factors: CEBPB, FOSL (FRA-1)1, FOXP3, GATA3, GATA4, HOXA10, HOXA3, ID2, IRF4, IRF8, MAF, NFATC1, NFATC2, NR4A1, NR4A3, POU2F2, REL, RELB, RORA, RORC, RUNX1 (AML1), RUNX3, STAT1, STAT4, STAT6, TOX, ZBTB7B. Epigenetically Regulated Genes: Th1 Cells: EOMES, IFNG, IL12RB2, IL18R1, IL18RAP, FASLG (TNFSF6), TBX21. Th2 Cells: ASB2, GATA3, IL13, IL1RL1, IL4, IL5, PPARG. Th17 Cells: IL17A, IL17RE, IL1R1, IL21, RORA, RORC. Inducible and Natural Regulatory T (iTreg and nTreg) Cells: CCR6, FOSL1 (FRA-1), FOXP3, IKZF2, IL9, IRF4, IRF8, MYB, NR4A1, NR4A3, POU2F2, REL, RELB, TGIF1, TNFSF11. Conventional Versus Regulatory T Cells: CACNA1F, CHD7, FOXP3, GATA4, HOPX, HOXA10, HOXA3, ID2, IKZF2, IL1R2, IL2RA, KIF2C, LRRC32, PERP, PKD2, TNFRSF9, TP53INP1, UTS2