Creative Biogene offers stable cell line development using an innovative and unique platform that allows one to go from gene to stable cell line. Based on many years' experience on cell line development, Creative Biogene's cell culture scientists focus on optimizing all parameters specific to your cell lines to efficiently produce stable cell lines according to your requirements.  Creative Biogene provides commercial and research cell line based on its proprietary expression vector, high throughput cell screening technologies and serum, animal components free process without any adaptation issue in a significantly reduced timeline.  Creative Biogene has developed innovative platforms ″Lenti-Stable″ (stable cell line using lentivector) and ″Select-Stable″ (stable cell line using selection system) to provide a rapid and cost-effective stable cell line service. Stable cell line is the best solution, not only to manufacture therapeutic and diagnostic protein, but also for application in drug screenings, as well as pharmacological and toxicological studies. Depending on the type of gene expression and the gene construct, there are many different approaches for establishing stable cell lines. Development of stable cell lines capable of producing recombinant proteins and monoclonal antibodies at high and reliable levels can be critical to a biopharmaceutical research project. The basis of manufacturing biologically active products relies on generating high quality cell lines which comply with current regulatory guidelines.  Creative Biogene platform provides a rapid and cost-effective solution for stable cell line generation, which can be used for cellular function assays, trafficking, protein production, target validation, and other research purposes.  Creative Biogene's expert staff and state-of-the-art facilities are fully equipped to execute complete studies on genetic and productive stability in the presence and absence of selective agents.  Creative Biogene "Select-Stable" platform uses Glutamine Synthetase (GS) or DihydrofolateReductase (DHFR) systems to construct the stable cell lines.

 • Generate the lentivirus particles. 
 • Sequentially transduce the cell line of your interest.
 • Select the clones constantly expressing your gene at high level.
 • Validation the genomic integration of gene via genomic PCR.
 • Validation the high-expression clone by Western Blot (if applicable).
 • Deliverable: Two cryogenic preserved vials of stable cells and final report.