cofilin,丝切蛋白抗体

产品编号HZ-2759R
英文名称cofilin
中文名称丝切蛋白抗体
别 名18 kDa phosphoprotein; CFL 1; CFL; CFL1; Cofilin 1; Cofilin 1 non muscle; Cofilin non muscle isoform; p18; COF1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 免疫学 生长因子和激素 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
cofilin,丝切蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量18kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原Synthetic peptide from the human cofilin conjugated to KLH
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
cofilin,丝切蛋白抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004].

Function:
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Subunit:
Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods.

Subcellular Location:
Nucleus matrix. Cytoplasm, cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.

cofilin,丝切蛋白抗体Tissue Specificity:
Widely distributed in various tissues.

Post-translational modifications:
Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells. Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.

Similarity:
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain.

Gene ID:
1072

Database links:
Entrez Gene: 1072 Human
Entrez Gene: 12631 Mouse
Entrez Gene: 29271 Rat
Omim: 601442 Human
SwissProt: P23528 Human
SwissProt: P18760 Mouse
SwissProt: P45592 Rat
Unigene: 170622 Human
Unigene: 329655 Mouse
Unigene: 11675 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

丝切蛋白(cofilin)是细胞骨架塑形的重要调节因素,其主要功能是分解肌动蛋白微丝和增加肌动蛋白单体从肌动蛋白微丝的末端解离的速度,从而促进肌动蛋白微丝的循环,是目前为止已发现的肌动蛋白结合蛋白家族中唯一能够改变肌动蛋白微丝扭曲结构的蛋白.

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-cofilin Polyclonal Antibody, Unconjugated(HZ-2759R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining