ARHI,抑癌基因ras同源家族1抗体

产品编号HZ-2903R
英文名称ARHI
中文名称抑癌基因ras同源家族1抗体
别 名DIRA3; DIRAS family GTP binding RAS like 3; Distinct subgroup of the Ras family member 3; GTP binding protein Di Ras3; NOEY2; Rho related GTP binding protein RhoI; RHOI; DIRA3_HUMAN.
规格价格0.1ml 0.2ml 
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 免疫学 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
ARHI,抑癌基因ras同源家族1抗体交叉反应Human,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量25kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ARHI
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ARHI,抑癌基因ras同源家族1抗体PubMedPubMed
产品介绍background:
ARHI contains a highly conserved GTP-binding domain, a putative effector domain distinct from that of RAS and RAP proteins, and a C-terminal membrane localization motif. ARHI mRNA is detected in all normal breast and ovarian epithelial cell cultures tested, as well as in normal ovary, heart, liver, pancreas and brain however ARHI expression is down-regulated in breast and ovarian tumors. Reactivation of ARHI expression in breast cancer cells is associated with increased histone H3 acetylation and decreased lysine 9 methylation of histone H3.

Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).

Tissue Specificity:
Expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers.

Similarity:
Belongs to the small GTPase superfamily. Di-Ras family.

Gene ID:
9077

ARHI,抑癌基因ras同源家族1抗体Database links:
Entrez Gene: 9077 Human
Omim: 605193 Human
SwissProt: O95661 Human
Unigene: 194695 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ARHI Polyclonal Antibody, Unconjugated(bs-2903R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining