CLASP1,细胞连接相关蛋白1抗体

产品编号HZ-7792R
英文名称CLASP1
中文名称细胞连接相关蛋白1抗体
别 名CLAP1_HUMAN; clasp1; CLIP associating protein 1; CLIP associating protein CLASP1; CLIP-associating protein 1; Cytoplasmic linker associated protein 1; Cytoplasmic linker-associated protein 1; hOrbit1; MAST1; Multiple asters 1; Multiple asters homolog 1; Protein Orbit homolog 1.
说 明 书0.1ml 0.2ml
研究领域细胞生物 细胞周期蛋白 细胞分化
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep,
CLASP1,细胞连接相关蛋白1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量169kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CLASP1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CLASP1,细胞连接相关蛋白1抗体PubMedPubMed
产品介绍background:
Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.

Function:
Microtubule plus-end tracking protein that promotes thestabilization of dynamic microtubules. Involved in the nucleationof noncentrosomal microtubules originating from the trans-Golginetwork (TGN). Required for the polarization of the cytoplasmicmicrotubule arrays in migrating cells towards the leading edge ofthe cell. May act at the cell cortex to enhance the frequency ofrescue of depolymerizing microtubules by attaching their plus-endsto cortical platforms composed of ERC1 and PHLDB2. This corticalmicrotubule stabilizing activity is regulated at least in part byphosphatidylinositol 3-kinase signaling. Also performs a similarstabilizing function at the kinetochore which is essential for thebipolar alignment of chromosomes on the mitotic spindle.

Subunit:
Interacts with CLIP2, ERC1, MAPRE1, MAPRE3, microtubules,PHLDB2 and RSN. The interaction with ERC1 may be mediated byPHLDB2. Interacts with GCC2; recruits CLASP1 to Golgi membranes.Interacts with MACF1 (By similarity).

Subcellular Location:
Cytoplasm, cytoskeleton. Cytoplasm,cytoskeleton, centrosome. Chromosome, centromere, kinetochore.Cytoplasm, cytoskeleton, spindle. Golgi apparatus, trans-Golginetwork (Probable). Note=Localizes to microtubule plus ends.Localizes to centrosomes, kinetochores and the mitotic spindle fromprometaphase. Subsequently localizes to the spindle midzone fromanaphase and to the midbody from telophase. In migrating cellslocalizes to the plus ends of microtubules within the cell body andto the entire microtubule lattice within the lamella. Localizes tothe cell cortex and this requires ERC1 and PHLDB2

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

CLASP1,细胞连接相关蛋白1抗体Similarity:
Belongs to the CLASP family.
Contains 7 HEAT repeats.

Database links:
UniProtKB/Swiss-Prot: Q7Z460.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.